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Modified Oligonucleotide Synthesis (final yield: μg scale)

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Modified Oligonucleotide Synthesis (final yield: μg scale)

Product Features

1. Seamless synthesis from small to large scale (µg–g range)

We offer flexible synthesis services, covering a wide range from small-scale (µg level) to large-scale (mg–g level) production.

2. Custom oligo synthesis using your own amidites

We can manufacture custom oligonucleotides using amidites you provide, including commercially available ones.
Before synthesis, we will work together with you to discuss and refine the production plan, ensuring careful communication and regular progress updates throughout the process.
Please feel free to contact us for details.

3. Extensive optional services

A variety of optional services are available to meet your specific needs, including in vivo grade products.
For more details, please visit our Optional Services page.

4. Wide selection of 63 modifications

We currently offer 63 types of modifications, with additional variations to be added in the future.
If you are looking for a modification that is not listed, we are happy to discuss custom modifications to meet your needs.
Our technical support team is ready to assist with your inquiries and requests — Please feel free to contact us anytime.

Click the modification name to see details (such as chemical structures).

Nucleic Acid Therapeutics – Screening and Animal studies

Extensive experience in manufacturing ASOs, dsRNAs, aptamers, etc.

No. Modification Position Purification
5′ Internal 3′ Cartridge HPLC
​ Phosphorothioate (PS) ✓ *1 ✓ ✓ *1 ✓ ✓
​ 2′-MOE ✓ ✓ ✓ ✓ ✓
​ 2′-F ✓ ✓ ✓ ✓ ✓
​ 2′-OMe ✓ ✓ ✓ ✓ ✓
​ 5-Methyl-dC ✓ ✓ ✓ ✓ ✓
​ Locked Nucleic Acid
 ✓ ✓ ✓ ✓ ✓
​ Acyclic Nucleic Acid (SNA) ✓ ✓ ✓ – ✓
​ Acyclic Nucleic Acid
(iL-aTNA) *2		 ✓ ✓ ✓ – ✓
  • *1  PS linkage can be inserted between the 5′ or 3′ modification and the oligonucleotide backbone.
  • *2  Available only under an agreement with Nagoya University. For inquiries, please contact us here.
Lipid and Ligand Conjugates – Drug Delivery System

Tri-GalNAc conjugation is utilized in seven approved oligonucleotide therapeutics, including Givlaari, Oxlumo, Leqvio, Amvuttra, Qfitlia, Wainua, and Tryngolza.

No. Modification Position Purification
5′ Internal 3′ Cartridge HPLC
​ Tri-GalNAcNEW – – ✓ – ✓
​ dR-GalNAc ✓ ✓ ✓ – ✓
​ GalNAc〈C3〉 ✓ ✓ ✓ – ✓
​ Cholesterol〈TEG〉 ✓ – ✓ – ✓
​ Tocopherol〈TEG〉 ✓ – – ✓ ✓
5′Phosphorylation – Ligation and 5′Modification of siRNA Guide Strands

Unmodified oligonucleotides from chemical synthesis have a 5’OH group.

No. Modification Position Purification
5′ Internal 3′ Cartridge HPLC
​ 5′Phosphate ✓ – – ✓ (RNA×) ✓
​ 5′Vinyl Phosphonate, 2′-OMe-U (5′VP)NEW ✓ – – – ✓
Fluorescent Dyes and Quenchers – Imaging and qPCR / dPCR Probes

No. Modification Position Purification λabs (nm) λem (nm) Compatible Quenchers
5′ Internal 3′ Cartridge HPLC
​ Alexa Fluor™ 488 *3NEW ✓ – ✓ – ✓ 494 517 BHQ-1 Dabcyl
​ Alexa Fluor™ 594 *3NEW ✓ – ✓ – ✓ 590 617 BHQ-2
​ Alexa Fluor™ 647 *3NEW ✓ – ✓ – ✓ 651 672 BHQ-3
​ Alexa Fluor™ 750 *3NEW ✓ – ✓ – ✓ 753 782 ー
​ ATTONEW ✓ – ✓ – ✓ ATTO-TEC Series List (PDF)
​ Fluorescein (6-FAM) ✓ ✓ *4 ✓ – *5 ✓ 494 525 Dabcyl TAMRA BHQ-1
​ Cyanine3 ✓ – ✓ – ✓ 552 565 BHQ-1 BHQ-2
​ TAMRA ✓ ✓ *4 ✓ – ✓ 555 580 BHQ-1 BHQ-2
​ Rhodamine (ROX) ✓ ✓ *4 ✓ – ✓ 568 595 BHQ-2
​ Cyanine5 ✓ – ✓ – ✓ 650 667 BHQ-2 BHQ-3
​ BHQ-1 – – ✓ – ✓ 534 – –
​ BHQ-2 – – ✓ – ✓ 579 – –
​ BHQ-3 – – ✓ – ✓ 672 – –
​ Dabcyl *6 – – ✓ – ✓ 453 – –
​ Real-Time PCR Probe *7 – – – – ✓ – – –
  • *3  Manufactured and sold under an intellectual property license from Life Technologies Corporation.
  • *4  Attached to dT via an amino linker.
  • *5  Cartridge purification is also available for 3′ or internal labeling.
  • *6  Ideal for molecular beacon applications.
  • *7  Catalog product (5′FAM-DNA-3′TAMRA) available for 11–30 mer oligos.

fluorescent-dyes-and-quenchers_wavelength-chart

Labeling – Detection and Purification

No. Modification Position Purification
5′ Internal 3′ Cartridge HPLC
​ Biotin〈standard〉 ✓ ✓ *4 – ✓ ✓
​ Biotin〈TEG〉 ✓ – ✓ ✓ ✓
​ DIG ✓ ✓ *4 ✓ – ✓
  • *4  Attached to dT via an amino linker.
Reactive Functional Groups – Conjugation

No. Modification Position Purification Reaction Partner Reaction Name Catalyst
5′ Internal 3′ Cartridge HPLC
​ Amino Modifier〈C6〉 ✓ – ✓ ✓ ✓ NHS Ester Amide Bond Formation Not Required
​ Amino-dT〈C6〉 ✓ ✓ ✓ ✓ ✓ NHS Ester Amide Bond Formation Not Required
​ EC Amino Linker ✓ – – ✓ ✓ NHS Ester Amide Bond Formation Not Required
​ Thiol *8 ✓ – ✓ – ✓ Maleimide Michael Addition Not Required
​ Azide ✓ ✓ *4 ✓ – ✓ Alkyne Click Chemistry Cu2+ *9
​ Alkynyl ✓ ✓ *4 ✓ – ✓ Azide Click Chemistry Cu2+
​ DBCO〈TEG〉 ✓ – – – ✓ Azide Click Chemistry Not Required
  • *4  Attached to dT via an amino linker.
  • *8  The 5′ modification has a C6 spacer and the 3′ modification has a C3 spacer.
  • *9  No catalyst is required when the counterpart is DBCO.
Terminators – DNA Polymerase Extension Inhibition

No. Modification Position Purification
5′ Internal 3′ Cartridge HPLC
​ 3′Phosphate – – ✓ ✓ ✓
​ 2′,3′-ddC – – ✓ ✓ ✓
​ idT – – ✓ ✓ ✓
Spacers

No. Modification Position Purification
5′ Internal 3′ Cartridge HPLC
​ Spacer C3 ✓ ✓ ✓ ✓ ✓
​ Spacer 9 ✓ ✓ ✓ ✓ ✓
​ Spacer C12 ✓ ✓ ✓ ✓ ✓
​ Spacer 18 ✓ ✓ ✓ ✓ ✓
​ d-Spacer ✓ ✓ ✓ ✓ ✓
​ r-Spacer ✓ ✓ ✓ ✓ ✓
Modified DNA Bases

No. Modification Position Purification
5′ Internal 3′ Cartridge HPLC
​ deoxyInosine (dI) ✓ ✓ ✓ ✓ ✓
​ deoxyUridine (dU) ✓ ✓ ✓ ✓ ✓
​ 5-hydroxymethyl-dC ✓ ✓ ✓ ✓ ✓
​ N6-Me-dA ✓ ✓ ✓ ✓ ✓
​ 8-oxo-dG ✓ ✓ ✓ – ✓
​ 8-oxo-dA ✓ ✓ ✓ – ✓
​ 5-Bromo-dU ✓ ✓ ✓ ✓ ✓
​ 8-Bromo-dA ✓ ✓ ✓ ✓ ✓
​ pseudo-dU ✓ ✓ ✓ ✓ ✓
​ 8-Amino-dA ✓ ✓ ✓ ✓ ✓
​ Reverse (Inverse) -Abasic ✓ ✓ ✓ ✓ ✓
Modified RNA Bases

No. Modification Position Purification
5′ Internal 3′ Cartridge HPLC
​ riboInosine (rI) ✓ ✓ ✓ ✓ ✓
​ 5-Methyl-rU (rT) ✓ ✓ ✓ ✓ ✓
​ pseudo-rU ✓ ✓ ✓ ✓ ✓
​ 2,6-diaminopurine (DAP) ✓ ✓ ✓ – ✓
Antibiotics

No. Modification Position Purification
5′ Internal 3′ Cartridge HPLC
​ puromycin – – ✓ ✓ ✓
Photo-Crosslinkers

No. Modification Position Purification
5′ Internal 3′ Cartridge HPLC
​ CNV-K ✓ ✓ ✓ ✓ ✓
​ CNV-D ✓ ✓ ✓ ✓ ✓

We also offer synthesis of various other modified oligonucleotides.

Selected examples include:

Phosphorodithioate (PS2), 1-Methyl-rA (m1A), 5-Methyl-rC (m5C), N6-Me-rA (m6A), 4-thio-rU (s4U) and others

Workflow - From Order to Delivery

  • Depending on when we receive your order, synthesis may begin before we send the delivery schedule notice.

We will send an Order Confirmation Notice by e-mail, so please make sure to review the order details (such as purification method, sequence, etc.).

  1. Place your order via our website or by e-mail.
  2. After receiving your order, we will inform you of the estimated delivery schedule by e-mail.
  3. Synthesis of your oligonucleotide will begin, followed by a thorough quality inspection.
  4. Prior to shipment, we conduct final quality checks and deliver only products that have passed our quality standards.

 

Order Acceptance and Shipping Information

Order cutoff time Mon–Sat E-mail / Web:  by 16:00
Shipping Mon–Sat In operation
Sun & Holidays Closed
Delivery Mon–Fri For inquiries, please use our inquiry form.
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