Nucleic Acid Extraction

• Service Description
• Nucleic Acid Extraction
• Samples
• Required Amount of Sample
• Price
• Ordering
• Cautions about Human Clinical Samples

 

Service Description

Nucleic acid is to be extracted from tissue, cell and blood, then purified.
Extracted and purified samples are subject to QC using tape station or bioanalyzer.

Nucleic Acid Extraction

– DNA extraction: genomic DNA
– RNA extraction: Total RNA

Samples

– Tissue, cell and blood

Required Amount of Sample

＜DNA Extraction＞

Sample Type	Approx. Required Amount	How to prepare	Container for transport
Tissue	square 3 mm on a side (approx. 30mg)	Freeze a tissue fragment instantly by using liquid nitrogen and store it at -80 degree C	1.5 or 2mL tube
Cell	approx. 106 cells	Freeze a cell pellet instantly by using liquid nitrogen and store it at -80 degree C
Blood	500uL or more	Collect blood using blood collection tube with anticoagulant agent, split the required amount and freeze it at -80 degree C

＜RNA Extraction＞

Sample Type	Approx. Required Amount	How to prepare	Container for transport
Tissue	square 3~5 mm on a side (approx. 30~100mg)	After RNAlater treatment*1 or freezing with liquid nitrogen instantly, store the tissue fragment at -80 degree C	1.5 or 2mL tube
Cell	approx. 106 cells	Freeze a cell pellet using liquid nitrogen instantly and store it at -80 degree C
Blood	2.5mL	Collect blood in PAXgene® Blood RNA Tube (2.5mL)*2, leave it at room temperature for at least 2 hours, and freeze it at -20 degree C for at least 24 hours	PAXgene®Bolld RNA Tube

Price

＜DNA / RNA Extraction＞

(Tax exclusive)

Number of Sample	Only for nucleic acid extraction service		Set price with analysis such as microarray and NGS
	Unit Price	Total Price	Unit Price	Total Price
1	46,000 JPY / sample	46,000 JPY	40,000 JPY / sample	40,000 JPY
2	34,000 JPY / sample	68,000 JPY	28,000 JPY / sample	56,000 JPY
3	31,000 JPY / sample	93,000 JPY	25,000 JPY / sample	75,000 JPY
4	26,000 JPY / sample	104,000 JPY	20,000 JPY / sample	80,000 JPY
5	23,000 JPY / sample	115,000 JPY	17,000 JPY / sample	85,000 JPY
6	21,000 JPY / sample	126,000 JPY	15,000 JPY / sample	90,000 JPY

Ordering

For Human clinical samples, please make sure to read through the“Cautions about human clinical samples”  described below .
The way of order is different between Human clinical samples and other samples.

Please inquire before shipping your samples.
Upon discussion with our staff, please send the sample(s) together with the order sheet to the below address;

Cautions about Human Clinical Samples

For Human clinical samples order, please make sure to read through the below descriptions.

< Sample Acceptability >

– We basically do not accept samples infected with pathogenetic microorganism or virus. Please refer to the below for details.

[ Unacceptable Samples ]

1. Samples infected with pathogenetic microorganisms of the bio safety level (BSL) 3 or higher according to National Institute of Infectious Diseases, or with infection possibility based on clinical test and observation
2. Samples infected with HCV or HBV viruses
3. Samples infected with other pathogenetic microorganism (BSL 2 or lower, BSL not determined) or viruses

Back to "Products & Services"