Guide for selecting purification grade

Here is the guide for selecting purification grade for your order.

Gene amplification G G G G G E
Sequencing primers N G G G G E
Gene amplification for subcloning *1 N G/I G G G E
Degenerated DNA *2 G G N N N N
Mutagenesis N N G G E E
Gene synthesis N N G I E I
Oligo longer than 51 bases N G E N E N
Oligo longer than 71 bases N N G N E N
Modification (terminal) N N G N N N
Modification (insertion) N I G N N N
Modification (Fluorescent) N N G N N N
Phosphorothioate Oligo (S-oligo) N N G G N N
E : Excellent
G : Good
 I  : Inquiry required (availability depends on sequence and modification)
N : Not recommended
*1 HPLC or PAGE purification are recommended for oligos longer than 30 bases or if require large amount of clones.
*2 Degenerated DNA means oligos with mixed base insertion.
– Please note that this table is only a guide without guarantee for your experiment. If anything wrong with using our product, please contact us.

Gel Filtration (11 - 50 bases)

Gel filtration takes off loose protecting group that comes off during the synthesis process. As a purification principle, it cannot clear out things with large molecular weight. Thus, synthesized DNA with defect base may remain. Also, short length oligos (less than 10 bases) may be resulted in low recovery rate.

Basically it is suitable for amplification of DNA fragment.

Reverse-phase Cartridge Purification (- 70 bases)

This has higher purity than gel filtration. However, the resin nature may make its separation power decreased for long oligos. Thus, we recommend it for oligo shorter than 70 bases.

It is suitable for primers for sequencing or PCR for subcloning.

LC-ESI-MS - Reverse-phase Cartridge Purification (- 50 bases)

This is the same purification as above but confirmed identity by LC-ESI-MS analysis. For LC-ESI-MS performance, this is a limited service for the product under 50 bases.

HPLC Purification (- 120 bases)

This is purification widely available for different purposes such as long oligos (over 30 bases) or modified oligos. Specially, many of the impurities produced on the synthesis process can be taken off. Oligos shorter than 50 bases are confirmed identity by LC-ESI-MS analysis after reverse-phase HPLC purification.

Pls. select this for high spec. experiment like mutagenesis or antisense.

IEX HPLC Purification (11 - 30 bases)

Oligos can be separated by base with this purification method, which makes it easier to separate the base defective oligos produced on the synthesis process than normal HPLC purification. As reverse-phase HPLC purification, this also confirms identity with LC-ESI-MS analysis. For higher grade purification, it is recommended to apply this purification after reverse-phase HPLC purification. (For detail, please see the “High Purification DNA” section.)

PAGE Purification (- 200 bases)

This highly purified oligo is purified by PAGE and then strictly quality controlled by LC-ESI-MS analysis (under 50 bases).
It is suitable for long DNA or artificial gene, etc.

High Purity DNA (20 - 50 bases)

Combined purifications are applied for this service: HPLC + IEX HPLC purifications for 20 – 30 bases oligos, and HPLC + PAGE purifications for 31 – 50 bases oligos. Such combined purifications enable average of 98% purity by taking off most of base defective products or other impurities. This is suitable for experiments which require highly purified oligos.

– LC-ESI-MS analysis is limited to product under 50 bases.

DNA Synthesis Option

RNase free option is available for your primers.
This is a suitable option for experiments under the presence of RNA, such as reverse
transcription reaction.

[Optional Service Price List]

(tax exclusive)
Optional Service
RNase free product
2,000 JPY
2 – 5 business days additionally to normal lead-time
RNase free product
20,000 JPY
* For service with QC, it is confirmed by QC test to be free of RNase activity.
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