Yield & Domestic Order Lead time

Yield Guarantee Non-modified DNA

Purification Synthesis Scale Available Length Comments
-10mers 11-50mers 51-70mers 71-99mers 100-120mers 121-150mers 151-200mers
Gel Filtration
Reverse-phase cartridge purification
OLIGOKIDS*6 1 OD Only gel filtration and reverse-phase cartridge purification are available for OLIGO KIDS scale oligos with the length between 11 and 30mers.
Gel Filteration*1 25nmol 2 OD Degenerated base*7 is available for more than 0.05µmol scale.
0.05µmol 5 OD
0.2µmol 8 OD
1µmol 30 OD
Reverse-phase cartridge purification*2

LC-ESI-MS-Reverse-phase cartridge purification*3

25nmol 1 OD Degenerated base*7 is available for more than 0.05µmol scale.
0.05µmol 3 OD
0.2µmol 1 OD 5 OD 5 OD
1µmol 5 OD 20 OD 20 OD
HPLC purification 25nmol 1 OD Degenerated base not available
0.05µmol 2 OD
0.2µmol 1 OD 3 OD 2 OD 1 OD
1µmol 4 OD 12 OD 6 OD 2 OD 1 OD
IEX HPLC purification*4 0.2µmol 1 OD Available only in 0.2 µmol scale

Degenerated base not available

High Purification*5 1µmol 1 OD Available only in 1µmol scale

Degenerated base not available

PAGE Purification 0.2µmol 1 OD 1 OD 1 OD Available only in 0.2µmol and 1µmol scale

Degenerated base not available

1µmol 4 OD 4 OD 2 OD 1 OD 1 OD 0.5 OD

*  Purification available for oligos less than 10mers are reverse-phase cartridge, LC-ESI-MS-reverse-phase cartridge and HPLC.
Also, for oligos between 71 and 120mers, HPLC or PAGE purification is available. For oligos between 121 and 200mers, only PAGE purification is available.

*1 Gel filtration is available for the oligos between 11 and 50mers.
*2 Reverse-phase cartridge purification is available for the oligos up to 70mers. HPLC purification or PAGE purification are recommended for the oligos longer than 71mers.
HSS will not be responsible for the orders other than this specification.
*3 LC-ESI-MS data attachment is available only for the oligos up to 50mers.
*4 IEX HPLC purification is available only for the oligos between 11 and 30mers.
*5 High purification is available only for the oligos between 20 and 50mers.
*6 More than two oligos order is required for OLIGO KIDS scale products. Only one oligo order is dealt as 25nmol scale product.
*7 HPLC and PAGE purifications are not available for oligos comprising degenerated base.

Please click here for how to select the adequate purification.

Yield Guarantee Modified DNA

Purification Synthesis Scale Available Length Comments
Up to 10mers 11-50mers 51-70mers 71-99mers 100-120mers 121-200mers
Reverse-phase cartridge purification*1 0.2µmol 1 OD 1 OD
1µmol 8 OD 4 OD
HPLC Purification 0.2µmol 1 OD 1 OD 1 OD Degenerated base not available
1µmol*2 8 OD 4 OD 2 OD Pl. inquire

* Orders for modified oligos are accepted in 0.2 µmol or more scale.
* Modified DNA is available only for HPLC purification except amination and phosphorylation which are available from Reverse-phase cartridge purification.
* Modification for 3′-end is available only for the oligos between 11 and 50mers.
* For applying more than one modification, please inquire separately.

*1 Reverse-phase cartridge purification is available for the oligos up to 70mers.
*2 Guaranteed yield for 1µmol scale phosphorothioate oligo is 15 OD

Please click here for detailed modification list.

Domestic Order Lead Time : Non-Modified DNA

Purification Available Length -10mers 11-50mers 51-70mers 71-99mers 100-120mers 121-200mers Comments
Gel filtration 11-50mers 1
Reverse-phase cartridge column purification -70mers 2 1 2
LC-ESI-MS-Reverse-phase cartridge column purification*1 -50mers 3 2 LC-ESI-MS data attachment is available only for the oligos up to 50mers.
HPLC purification -120mers 3-4 2-3 3-4 Degenerated base not available
IEX HPLC purification*2 11-30mers 3-4 Available only in 0.2µmol scale

Degenerated base not available
High purification*3 20-50mers 10 Available only in 1µmol scale

Degenerated base not available
PAGE purification 11-200mers 6 7-12 9-14 Available only in 0.2µmol or more scale

Degenerated base not available

* Indicated lead time is the number of business days. (The order date is counted as “0” day.)
* For the orders of 1µmol or more scale, extra one day will be required to the above turnaround.
* Longer lead time may be required for more than 20 oligos orders as well as depending on sequence organization.
* HSS will not be responsible for the orders other than the available length in the above table.
* Purification available for oligos less than 10mers are reverse-phase cartridge, LC-ESI-MS-reverse-phase cartridge and HPLC.
Also, for oligos between 71 and 120mers, HPLC or PAGE purification is available, and for oligos between 121 and 200mers, only PAGE is available.

*1 LC-ESI-MS-Reverse-phase cartridge purification is available for the oligos up to 50mers.
*2 IEX HPLC purification is available only for the oligos between 11 and 30mers.
*3 High purification is available only for the oligos between 20 and 50mers.

Please click here for how to select the adequate purification.

Domestic Order Lead Time : Modified DNA

Purification Available Length -10mers 11-50mers 51-70mers 71-99mers 100-120mers 121-200mers Comments
Reverse-phase cartridge column purification 11-70mers 7-12 9-14 Please inquire.
HPLC purification 11-120mers Degenerated base not available

* Indicated lead time is the number of business days. (The order date is counted as “0” day.)
* For the orders of 1µmol or more scale, extra one day will be required to the above turnaround.
* Longer lead time may be required for more than 20 oligos orders as well as depending on sequence organization.
* Modified DNA is available only for HPLC purification except amination and phosphorylation which are available from Reverse-phase cartridge purification.
* Modification for 3′-end is available only for the oligos between 11 and 50mers.
* Delivery products can be dispensed. Please inquire.

Please refer to the Modification List for detail.

Domestic Order Lead Time : Large Amount DNA Synthesis

Yield Guarantee Non-Modified DNA Modified DNA Phosphorothioate DNA
1-2mg 7-12 Please inquire. 7-12
3-5mg 7-12 7-12
10mg 9-14 9-14

* Indicated lead time is the number of business days. (The order date is counted as “0” day.)
* Purification available for miligram orders is only HPLC.
* Please inquire on the oligo more than 10mg.

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