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# Modification

### Synthesis

#### Modification List

Modification |
DNA Synthesis |
RNA Synthesis |
Notes |
||

Reverse-phase cartridge purification |
HPLC purification |
Reverse-phase cartridge purification |
HPLC purification |
||

5′ Amination (C6) | A | A | A | A | Structural formula |

3′ Amination (C6) | A | A | A | A | Structural formula |

5′-EC Amino Linker | A | A | A | A | Structural formula |

Amino(C6)-dT insertion | A | A | A | A | Structural formula |

5′ Biotin | N/A | A | N/A | A | Structural formula |

3′ Biotin | N/A | A | N/A | A | Structural formula |

5’Biotin(TEG) | A | A | A | A | Structural formula |

5′ Cyanine3 | N/A | A | N/A | A | Structural formula |

5′ Cyanine5 | N/A | A | N/A | A | Structural formula |

3’Cyanine3 | N/A | A | N/A | A | Structural formula |

3’Cyanine5 | N/A | A | N/A | A | Structural formula |

5′ Phosphorylation | A | A | N/A | A | Structural formula |

3′ Phosphorylation | A | A | A | A | Structural formula |

5′ FITC (6-FAM) | N/A | A | N/A | A | Structural formula |

3′ FITC (6-FAM) | N/A | A | N/A | A | Structural formula |

Fluorescein-dT insertion | N/A | A | N/A | A | Structural formula |

5′ TAMRA | N/A | A | N/A | A | Structural formula |

3′ TAMRA | N/A | A | N/A | A | Structural formula |

5′ Rhodamine (ROX) | N/A | A | N/A | A | Structural formula |

3′ Rhodamine (ROX) | N/A | A | N/A | A | Structural formula |

3′ BHQ1 | N/A | A | N/A | A | Structural formula |

3′ BHQ2 | N/A | A | N/A | A | Structural formula |

3′ BHQ3 | N/A | A | N/A | A | Structural formula |

Real time PCR probe | N/A | A | N/A | N/A | Structural formula |

5′ Cholesterol | N/A | A | N/A | A | Structural formula |

3′ Cholesterol | N/A | A | N/A | A | Structural formula |

3′ idT | A | A | A | A | Structural formula |

5′ Thiol (C6) | N/A | A | N/A | A | Structural formula |

3′ Thiol (C3) | N/A | A | N/A | A | Structural formula |

5′ Azide | N/A | A | N/A | A | Structural formula |

3′ Azide | N/A | A | N/A | A | Structural formula |

5′ Alkynyl | N/A | A | N/A | A | Structural formula |

3′ Alkynyl | N/A | A | N/A | A | Structural formula |

5’DBCO(TEG) | N/A | A | N/A | A | Structural formula |

3′-(2′,3′-ddC) | A | A | A | A | Structural formula |

3’Dabcyl | N/A | A | N/A | A | Structural formula |

Spacer C3 | A | A | A | A | Structural formula |

Spacer C12 | A | A | A | A | Structural formula |

Spacer 9 | A | A | A | A | Structural formula |

Spacer 18 | A | A | A | A | Structural formula |

Uracil insertion | A | A | A | A | Structural formula |

Inosine insertion | A | A | A | A | Structural formula |

Phosphorothioated | N/A | A | N/A | A | Structural formula |

d-Spacer | A | A | A | A | Structural formula |

r-Spacer | N/A | N/A | N/A | A | Structural formula |

RNA-T (ribothymidine) insertion | N/A | N/A | N/A | A | Structural formula |

Methylene Bicyclo Nucleic acid | A | A | A | A | Structural formula |

Artificial acyclic nucleic acid (SNA) | N/A | A | N/A | A | Structural formula |

Artificial acyclic nucleic acid (iL-aTNA) | N/A | A | N/A | A | Structural formula |

2′-O-Methylated RNA insertion | A | A | A | A | Structural formula |

2′-Fluoro RNA insertion | A | A | A | A | Structural formula |

2′-O-MOE insertion | A | A | A | A | Structural formula |

5-Methyl-dC insertion | A | A | A | A | Structural formula |

8-Bromo-dA insertion | A | A | A | A | Structural formula |

5-Bromo-dU insertion | A | A | A | A | Structural formula |

pseudo-dU insertion | N/A | A | N/A | A | Structural formula |

pseudo-rU insertion | N/A | A | N/A | A | Structural formula |

N6-Me-dA | N/A | A | N/A | A | Structural formula |

8-oxo-dG | N/A | A | N/A | A | Structural formula |

8-oxo-dA | N/A | A | N/A | A | Structural formula |

8-Amino-dA | N/A | A | N/A | A | Structural formula |

Inosine (RNA) | N/A | N/A | N/A | A | Structural formula |

2-Amino-A (= 2,6-diaminopurine) (RNA) | N/A | A | N/A | A | Structural formula |

Photocrosslinkable oligo CNV series (CNV-K) | A | A | A | A | Structural formula |

Photocrosslinkable oligo CNV series (CNV-D) | A | A | A | A | Structural formula |

* For modification not listed above, please inquire.

* Modification for 3′-end is available only for the oligos between 11 and 50 bases.