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- Rhodococcus erythropolis Protein Expression System
Rhodococcus erythropolis Protein Expression System
- Rhodococcus erythropolis L88 Competent Cells
- Rhodococcus Expression Vectors pTip, pNit, pCpi Vector Series
Because Rhodococcus erythropolis L88 Competent Cells can grow and express recombinant protein at 4 to 30 °C, it is expected to successfully express lethal protein, protein inhibiting cell proliferation, and protein forming inclusion body.
It is realized by the combination of Rhodococcus erythropolis L88 Competent Cells and pTip, pNit,pCpi Vector Series.
| These products are produced and sold by Hokkaido System Science Co., Ltd. with the license granted from National Institute of Advanced Industrial Science and Technology (AIST). |
Rhodococcus erythropolis L88 Competent Cells
Production Specification
Rhodococcus erythropolis L88 is a lysozyme-sensitive strain which was obtained by inducing point mutation through UV irradiation to Rhodococcus erythropolis JCM3201. Wild type is resistant to over 1 mg/mL lysozyme, whereas 12.5 µg/mL lysozyme cause bacteriolysis to Rhodococcus erythropolis L88. Therefore, it is easier to disrupt cells and extract protein than wild type.
This strain can grow and express recombinant protein at 4 to 30 °C.
Price （tax exclusive）
| Product No. | Product Name | Content | Price |
| RE-L88 | Rhodococcus erythropolis L88 Competent Cells | 500 µL (100 µL × 5) |
20,000 JPY |
How to order
| It is necessary to submit “Rhodococcus erythropolis Protein Expression System License Agreement” when you order these products. Please E-mail the order form and License agreement to the address below. |
【E-mail】
goods@hssnet.co.jp
Please inquire for details.
Rhodococcus Expression Vectors pTip, pNit, pCpi Vector Series
Production Specification
Regulation of expression level and co-expression are workable with the combination of three promoters (thiostrepton-induced pTip, moderate expression pNit, and high expression pCpi promoters) and two replication origins (theta and rolling circle-types).
Rhodococcus erythropolis L88 Competent Cells (Product No. RE-L88) is applicable to expression of induced gene. Transformation efficiency is 1×104 colonies/µg at least.
pTip, pNit, pCpi Vector Series are shuttle vectors and can be applied to E. coli in cloning and vector construction; copy number is 300-500.
Since two His-tag sequences are in multi cloning sites, it is easy to design His-tagged recombinant protein and refine it.
Vector maps
Multi Cloning Sites
Price （tax exclusive）
pTip, pNit, pCpi Vector Series.
| Product No. | Product Name | Mass | Concentration | Price |
| RE-0001 ~ RE-0024 |
Please see the list below. | 10 µg | 100 ng / µL | 32,000 JPY |
List of Vectors
| Product No. |
Product Name |
Promoter | Selection | Replication | MCS | Size (bp) |
Accession |
| RE-0001 | pTipQT1 | tipA (inducible) | tetracycline | theta type | type1 | 8,207 | AB127592.1 |
| RE-0002 | pTipQT2 | tipA (inducible) | tetracycline | theta type | type2 | 8,211 | AB127593.1 |
| RE-0003 | pTipRT1 | tipA (inducible) | tetracycline | rolling circle | type1 | 8,275 | AB127596.1 |
| RE-0004 | pTipRT2 | tipA (inducible) | tetracycline | rolling circle | type2 | 8,279 | AB127597.1 |
| RE-0005 | pTipQC1 | tipA (inducible) | chloramphenicol | theta type | type1 | 8,343 | AB127590.1 |
| RE-0006 | pTipQC2 | tipA (inducible) | chloramphenicol | theta type | type2 | 8,388 | AB127591.1 |
| RE-0007 | pTipRC1 | tipA (inducible) | chloramphenicol | rolling circle | type1 | 8,452 | AB127594.1 |
| RE-0008 | pTipRC2 | tipA (inducible) | chloramphenicol | rolling circle | type2 | 8,456 | AB127595.1 |
| RE-0009 | pNitQT1 | nit (constitutive) |
tetracycline | theta type | type1 | 5,984 | AB127600.1 |
| RE-0010 | pNitQT2 | nit (constitutive) |
tetracycline | theta type | type2 | 5,988 | AB127601.1 |
| RE-0011 | pNitRT1 | nit (constitutive) |
tetracycline | rolling circle | type1 | 6,058 | AB127604.1 |
| RE-0012 | pNitRT2 | nit (constitutive) |
tetracycline | rolling circle | type2 | 6,062 | AB127605.1 |
| RE-0013 | pNitQC1 | nit (constitutive) |
chloramphenicol | theta type | type1 | 6,153 | AB127598.1 |
| RE-0014 | pNitQC2 | nit (constitutive) |
chloramphenicol | theta type | type2 | 6,157 | AB127599.1 |
| RE-0015 | pNitRC1 | nit (constitutive) |
chloramphenicol | rolling circle | type1 | 6,227 | AB127602.1 |
| RE-0016 | pNitRC2 | nit (constitutive) |
chloramphenicol | rolling circle | type2 | 6,231 | AB127603.1 |
| RE-0017 | pCpiQT1 | Cpi (constitutive) |
tetracycline | theta type | type1 | 5,923 | – |
| RE-0018 | pCpiQT2 | Cpi (constitutive) |
tetracycline | theta type | type2 | 5,926 | – |
| RE-0019 | pCpiRT1 | Cpi (constitutive) |
tetracycline | rolling circle | type1 | 5,997 | – |
| RE-0020 | pCpiRT2 | Cpi (constitutive) |
tetracycline | rolling circle | type2 | 6,000 | – |
| RE-0021 | pCpiQC1 | Cpi (constitutive) |
chloramphenicol | theta type | type1 | 6,092 | – |
| RE-0022 | pCpiQC2 | Cpi (constitutive) |
chloramphenicol | theta type | type2 | 6,095 | – |
| RE-0023 | pCpiRC1 | Cpi (constitutive) |
chloramphenicol | rolling circle | type1 | 6,166 | – |
| RE-0024 | pCpiRC2 | Cpi (constitutive) |
chloramphenicol | rolling circle | type2 | 6,169 | – |
How to order
It is necessary to submit “Rhodococcus erythropolis Protein Expression System License Agreement” when you order these products.
Please E-mail the order form and License agreement to the address below.
【E-mail】
goods@hssnet.co.jp
Please inquire for details.
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