Rhodococcus erythropolis Protein Expression System

Because Rhodococcus erythropolis L88 Competent Cells can grow and express recombinant protein at 4 to 30 °C, it is expected to successfully express lethal protein, protein inhibiting cell proliferation, and protein forming inclusion body.
It is realized by the combination of Rhodococcus erythropolis L88 Competent Cells and pTip, pNit,pCpi Vector Series.

These products are produced and sold by Hokkaido System Science Co., Ltd. with the license granted from National Institute of Advanced Industrial Science and Technology (AIST).

Rhodococcus erythropolis L88 Competent Cells

Production Specification

Rhodococcus erythropolis L88 is a lysozyme-sensitive strain which was obtained by inducing point mutation through UV irradiation to Rhodococcus erythropolis JCM3201. Wild type is resistant to over 1 mg/ml lysozyme, whereas 12.5 µg/ml lysozyme cause bacteriolysis to Rhodococcus erythropolis L88. Therefore, it is easier to disrupt cells and extract protein than wild type.
This strain can grow and express recombinant protein at 4 to 30 °C.

Datasheet download

Price (tax exclusive)

Product No. Product Name Content Price
RE-L88 Rhodococcus erythropolis L88 Competent Cells 500 µl
(100 µl × 5)
20,000 JPY

How to order

It is necessary to submit “Rhodococcus erythropolis Protein Expression System License Agreement” when you order these products.
Please E-mail the order form and License agreement to the address below.

Order Form download

License Agreement

【E-mail】
goods@hssnet.co.jp
Please inquire for details.

 

Rhodococcus Expression Vectors pTip, pNit, pCpi Vector Series

Production Specification

Regulation of expression level and co-expression are workable with the combination of three promoters (thiostrepton-induced pTip, moderate expression pNit, and high expression pCpi promoters) and two replication origins (theta and rolling circle-types).
Rhodococcus erythropolis L88 Competent Cells (Product No. RE-L88) is applicable to expression of induced gene. Transformation efficiency is 1×104 colonies/µg at least.
pTip, pNit, pCpi Vector Series are shuttle vectors and can be applied to E. coli in cloning and vector construction; copy number is 300-500.
Since two His-tag sequences are in multi cloning sites, it is easy to design His-tagged recombinant protein and refine it.

Datasheet download

 Vector maps

 

 Multi Cloning Sites

 

Price (tax exclusive)

pTip, pNit, pCpi Vector Series.

Product No. Product Name Mass Concentration Price
RE-0001
~
RE-0024
Please see the list below. 10 µg 100 ng / µl 32,000 JPY

 List of Vectors

Product
No.
Product
Name
Promoter Selection Replication MCS Size
(bp)
Accession
RE-0001 pTipQT1 tipA (inducible) tetracycline theta type type1 8,207 AB127592.1
RE-0002 pTipQT2 tipA (inducible) tetracycline theta type type2 8,211 AB127593.1
RE-0003 pTipRT1 tipA (inducible) tetracycline rolling circle type1 8,275 AB127596.1
RE-0004 pTipRT2 tipA (inducible) tetracycline rolling circle type2 8,279 AB127597.1
RE-0005 pTipQC1 tipA (inducible) chloramphenicol theta type type1 8,343 AB127590.1
RE-0006 pTipQC2 tipA (inducible) chloramphenicol theta type type2 8,388 AB127591.1
RE-0007 pTipRC1 tipA (inducible) chloramphenicol rolling circle type1 8,452 AB127594.1
RE-0008 pTipRC2 tipA (inducible) chloramphenicol rolling circle type2 8,456 AB127595.1
RE-0009 pNitQT1 nit
(constitutive)
tetracycline theta type type1 5,984 AB127600.1
RE-0010 pNitQT2 nit
(constitutive)
tetracycline theta type type2 5,988 AB127601.1
RE-0011 pNitRT1 nit
(constitutive)
tetracycline rolling circle type1 6,058 AB127604.1
RE-0012 pNitRT2 nit
(constitutive)
tetracycline rolling circle type2 6,062 AB127605.1
RE-0013 pNitQC1 nit
(constitutive)
chloramphenicol theta type type1 6,153 AB127598.1
RE-0014 pNitQC2 nit
(constitutive)
chloramphenicol theta type type2 6,157 AB127599.1
RE-0015 pNitRC1 nit
(constitutive)
chloramphenicol rolling circle type1 6,227 AB127602.1
RE-0016 pNitRC2 nit
(constitutive)
chloramphenicol rolling circle type2 6,231 AB127603.1
RE-0017 pCpiQT1 Cpi
(constitutive)
tetracycline theta type type1 5,923
RE-0018 pCpiQT2 Cpi
(constitutive)
tetracycline theta type type2 5,926
RE-0019 pCpiRT1 Cpi
(constitutive)
tetracycline rolling circle type1 5,997
RE-0020 pCpiRT2 Cpi
(constitutive)
tetracycline rolling circle type2 6,000
RE-0021 pCpiQC1 Cpi
(constitutive)
chloramphenicol theta type type1 6,092
RE-0022 pCpiQC2 Cpi
(constitutive)
chloramphenicol theta type type2 6,095
RE-0023 pCpiRC1 Cpi
(constitutive)
chloramphenicol rolling circle type1 6,166
RE-0024 pCpiRC2 Cpi
(constitutive)
chloramphenicol rolling circle type2 6,169

How to order

It is necessary to submit “Rhodococcus erythropolis Protein Expression System License Agreement” when you order these products.
Please E-mail the order form and License agreement to the address below.

Order Form download

License Agreement

【E-mail】
goods@hssnet.co.jp
Please inquire for details.

 

Back to "Products & Services"
Page Top