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Modified Oligonucleotide Synthesis

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Product Features

1. Seamless synthesis from small to large scale (µg–g range)

We offer flexible synthesis services, covering a wide range from small-scale (µg level) to large-scale (mg–g level) production.

2. Custom oligo synthesis using your own amidites

We can manufacture custom oligonucleotides using amidites you provide, including commercially available ones.
Before synthesis, we will work together with you to discuss and refine the production plan, ensuring careful communication and regular progress updates throughout the process.
Please feel free to contact us for details.

3. Extensive optional services

A variety of optional services are available to meet your specific needs, including in vivo grade products.
For more details, please visit our Optional Services page.

4. Wide selection of 63 modifications

We currently offer 63 types of modifications, with additional variations to be added in the future.
If you are looking for a modification that is not listed, we are happy to discuss custom modifications to meet your needs.
Our technical support team is ready to assist with your inquiries and requests — Please feel free to contact us anytime.
In the list below, you can click on each modification name to view its chemical structure.

Nucleic Acid Therapeutics – Screening and Animal studies

Extensive experience in manufacturing ASOs, dsRNAs, aptamers, etc.

No. Modification Position Purification
5′ Internal 3′ Cartridge HPLC
1 Phosphorothioate (PS) ✓ *1 ✓ ✓ *1 ✓ ✓
2 2′-MOE ✓ ✓ ✓ ✓ ✓
3 2′-F ✓ ✓ ✓ ✓ ✓
4 2′-OMe ✓ ✓ ✓ ✓ ✓
5 5-Methyl-dC ✓ ✓ ✓ ✓ ✓
6 Locked Nucleic Acid
 ✓ ✓ ✓ ✓ ✓
7 Acyclic Nucleic Acid (SNA) ✓ ✓ ✓ – ✓
8 Acyclic Nucleic Acid
(iL-aTNA) *2		 ✓ ✓ ✓ – ✓
  • *1  PS linkage can be inserted between the 5′ or 3′ modification and the oligonucleotide backbone.
  • *2  Available only under an agreement with Nagoya University. For inquiries, please contact us here.
Lipid and Ligand Conjugates – Drug Delivery System

Tri-GalNAc conjugation is utilized in seven approved oligonucleotide therapeutics, including Givlaari, Oxlumo, Leqvio, Amvuttra, Qfitlia, Wainua, and Tryngolza.

No. Modification Position Purification
5′ Internal 3′ Cartridge HPLC
9 Tri-GalNAc　【NEW】 – – ✓ – ✓
10 dR-GalNAc ✓ ✓ ✓ – ✓
11 GalNAc〈C3〉 ✓ ✓ ✓ – ✓
12 Cholesterol〈TEG〉 ✓ – ✓ – ✓
13 Tocopherol〈TEG〉 ✓ – – ✓ ✓
5′Phosphorylation – Ligation and 5′Modification of siRNA Guide Strands

Unmodified oligonucleotides from chemical synthesis have a 5’OH group.

No. Modification Position Purification
5′ Internal 3′ Cartridge HPLC
14 5′Phosphate ✓ – – ✓ (RNA×) ✓
15 5′Vinyl Phosphonate, 2′-OMe-U (5′VP)【NEW】 ✓ – – – ✓
Fluorescent Dyes and Quenchers – Imaging and qPCR / dPCR Probes

Also applicable as probes for virus detection and other diagnostic purposes.

No. Modification Position Purification λabs (nm) λem (nm) Compatible Quenchers
5′ Internal 3′ Cartridge HPLC
16 Fluorescein (6-FAM) ✓ ✓ *3 ✓ – *4 ✓ 494 525 Dabcyl TAMRA BHQ-1
17 Cyanine3 ✓ – ✓ – ✓ 552 565 BHQ-1 BHQ-2
18 TAMRA ✓ ✓ *3 ✓ – ✓ 555 580 BHQ-1 BHQ-2
19 Rhodamine (ROX) ✓ ✓ *3 ✓ – ✓ 568 595 BHQ-2
20 Cyanine5 ✓ – ✓ – ✓ 650 667 BHQ-2 BHQ-3
21 BHQ-1 – – ✓ – ✓ 534 – –
22 BHQ-2 – – ✓ – ✓ 579 – –
23 BHQ-3 – – ✓ – ✓ 672 – –
24 Dabcyl *5 – – ✓ – ✓ 453 – –
25 Real-Time PCR Probe *6 – – – – ✓ – – –
  • *3  Attached to dT via an amino linker.
  • *4  Cartridge purification is also available for 3′ or internal labeling.
  • *5  Ideal for molecular beacon applications.
  • *6  Catalog product (5′FAM-DNA-3′TAMRA) available for 11–30 mer oligos.

ATTO-TEC Fluorescent Dyes – Imaging and qPCR / dPCR Probes

ATTO-TEC series covers a wide spectral range from visible to near-infrared (390–740 nm) and can also be used as alternatives to various other fluorescent dyes.

No. Modification Position Purification λabs (nm) λem (nm) Compatible Quenchers
5′ Internal 3′ Cartridge HPLC
26 ATTO 【NEW】 ✓ – ✓ – ✓ ATTO-TEC Series List (PDF)
Labeling – Detection and Purification

No. Modification Position Purification
5′ Internal 3′ Cartridge HPLC
27 Biotin〈standard〉 ✓ ✓ *3 – ✓ ✓
28 Biotin〈TEG〉 ✓ – ✓ ✓ ✓
29 DIG ✓ ✓ *3 ✓ – ✓
  • *3  Attached to dT via an amino linker.
Reactive Functional Groups – Conjugation

No. Modification Position Purification Reaction Partner Reaction Name Catalyst
5′ Internal 3′ Cartridge HPLC
30 Amino Modifier〈C6〉 ✓ – ✓ ✓ ✓ NHS Ester Amide Bond Formation Not Required
31 Amino-dT〈C6〉 ✓ ✓ ✓ ✓ ✓ NHS Ester Amide Bond Formation Not Required
32 EC Amino Linker ✓ – – ✓ ✓ NHS Ester Amide Bond Formation Not Required
33 Thiol *7 ✓ – ✓ – ✓ Maleimide Michael Addition Not Required
34 Azide ✓ ✓ *3 ✓ – ✓ Alkyne Click Chemistry Cu2+ *8
35 Alkynyl ✓ ✓ *3 ✓ – ✓ Azide Click Chemistry Cu2+
36 DBCO〈TEG〉 ✓ – – – ✓ Azide Click Chemistry Not Required
  • *3  Attached to dT via an amino linker.
  • *7  The 5′ modification has a C6 spacer and the 3′ modification has a C3 spacer.
  • *8  No catalyst is required when the counterpart is DBCO.
Terminators – DNA Polymerase Extension Inhibition

No. Modification Position Purification
5′ Internal 3′ Cartridge HPLC
37 3′Phosphate – – ✓ ✓ ✓
38 2′,3′-ddC – – ✓ ✓ ✓
39 idT – – ✓ ✓ ✓
Spacers

No. Modification Position Purification
5′ Internal 3′ Cartridge HPLC
40 Spacer C3 ✓ ✓ ✓ ✓ ✓
41 Spacer 9 ✓ ✓ ✓ ✓ ✓
42 Spacer C12 ✓ ✓ ✓ ✓ ✓
43 Spacer 18 ✓ ✓ ✓ ✓ ✓
44 d-Spacer ✓ ✓ ✓ ✓ ✓
45 r-Spacer ✓ ✓ ✓ ✓ ✓
Modified DNA Bases

No. Modification Position Purification
5′ Internal 3′ Cartridge HPLC
46 deoxyInosine (dI) ✓ ✓ ✓ ✓ ✓
47 deoxyUridine (dU) ✓ ✓ ✓ ✓ ✓
48 5-hydroxymethyl-dC ✓ ✓ ✓ ✓ ✓
49 N6-Me-dA ✓ ✓ ✓ ✓ ✓
50 8-oxo-dG ✓ ✓ ✓ – ✓
51 8-oxo-dA ✓ ✓ ✓ – ✓
52 5-Bromo-dU ✓ ✓ ✓ ✓ ✓
53 8-Bromo-dA ✓ ✓ ✓ ✓ ✓
54 pseudo-dU ✓ ✓ ✓ ✓ ✓
55 8-Amino-dA ✓ ✓ ✓ ✓ ✓
56 Reverse (Inverse) -Abasic ✓ ✓ ✓ ✓ ✓
Modified RNA Bases

No. Modification Position Purification
5′ Internal 3′ Cartridge HPLC
57 riboInosine (rI) ✓ ✓ ✓ ✓ ✓
58 5-Methyl-rU (rT) ✓ ✓ ✓ ✓ ✓
59 pseudo-rU ✓ ✓ ✓ ✓ ✓
60 2,6-diaminopurine (DAP) ✓ ✓ ✓ – ✓
Antibiotics

No. Modification Position Purification
5′ Internal 3′ Cartridge HPLC
61 puromycin – – ✓ ✓ ✓
Photo-Crosslinkers

No. Modification Position Purification
5′ Internal 3′ Cartridge HPLC
62 CNV-K ✓ ✓ ✓ ✓ ✓
63 CNV-D ✓ ✓ ✓ ✓ ✓

We also offer synthesis of various other modified oligonucleotides.

Selected examples include:

Phosphorodithioate (PS2), 1-Methyl-rA (m1A), 5-Methyl-rC (m5C), N6-Me-rA (m6A), 4-thio-rU (s4U) and others

Workflow - From Order to Delivery

* Depending on when we receive your order, synthesis may begin before we send the delivery schedule notice.

We will send an Order Confirmation Notice by e-mail, so please make sure to review the order details (such as purification method, sequence, etc.).

1. Place your order via our website or by e-mail.

2. After receiving your order, we will inform you of the estimated delivery schedule by e-mail.

3. Synthesis of your oligonucleotide will begin, followed by a thorough quality inspection.

4. Prior to shipment, we conduct final quality checks and deliver only products that have passed our quality standards.

 

Order Acceptance and Shipping Information

Order cutoff time Mon–Sat E-mail / Web:  by 16:00
Shipping Mon–Sat In operation
Sun & Holidays Closed
Delivery Mon–Fri For inquiries, please use our inquiry form.
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