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IP pro-Taq DNA Polymerase
Product Description
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Brand | LaboPass |
| Category | DNA Polymerase | |
| Product Name | IP pro-Taq DNA Polymerase | |
| Storage Conditions | -20°C | |
| Download | Protocol  |
| Cat.No. | Product Description | Size (unit) | Price(JPY) |
| CMT2001 | LaboPass™ IP pro-Taq DNA Polymerase (2.5 u/μl) | 250 | 17,500 |
| CMT2002 | LaboPass™ IP pro-Taq DNA Polymerase (2.5 u/μl) | 500 | 30,000 |
| CMT2004 | LaboPass™ IP pro-Taq DNA Polymerase (2.5 u/μl) | 1,000 | 59,000 |
| CMT2301 | Mega3 LaboPass™ IP pro-Taq DNA Polymerase (2.5 u/μl) | 250×3 | 49,000 |
| CMT2302 | Mega3 LaboPass™ IP pro-Taq DNA Polymerase (2.5 u/μl) | 500×3 | 84,000 |
| CMT2304 | Mega3 LaboPass™ IP pro-Taq DNA Polymerase (2.5 u/μl) | 1,000×3 | 165,000 |
| CMT2501 | Mega5 LaboPass™ IP pro-Taq DNA Polymerase (2.5 u/μl) | 250×5 | 80,000 |
| CMT2502 | Mega5 LaboPass™ IP pro-Taq DNA Polymerase (2.5 u/μl) | 500×5 | 137,000 |
| CMT2504 | Mega5 LaboPass™ IP pro-Taq DNA Polymerase (2.5 u/μl) | 1,000×5 | 268,000 |
| CMT21001 | Mega10 LaboPass™ IP pro-Taq DNA Polymerase (2.5 u/μl) | 250×10 | 154,000 |
| CMT21002 | Mega10 LaboPass™ IP pro-Taq DNA Polymerase (2.5 u/μl) | 500×10 | 264,000 |
| CMT21004 | Mega10 LaboPass™ IP pro-Taq DNA Polymerase (2.5 u/μl) | 1,000×10 | 519,000 |
LaboPass™ IP pro-Taq DNA Polymerase is a modified version of Taq polymerase which improves the reliability and specificity of PCR reaction. The IP pro-Taq Polymerase has proofreading activity and is more thermostable than wild type Taq DNA polymerase, which allows for the amplification of long length up to 20 kb with high accuracy. The amplified products contain a mixture of blunt ends and 3′ A-plus ends.
Applications
-General PCR for detection
-Long range PCR
-Real-time PCR
-TA-cloning
-Blunt-end cloning
Supplied Reagents
LaboPass™ IP pro- Taq DNA Polymerase is provided with an optimized buffer to improved PCR yield.
| 10X IP pro-Taq buffer (with MgCl2) LaboPass™ IP pro-Taq DNA Polymerase is supplied with an optimized reaction buffer to improve PCR yield. |
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5X Tuning buffer
Tuning buffer can improve PCR efficiency in reaction using problematic template DNA containing high GC contents or stable secondary structure. Thus, it is advantageous to amplify complicated long target sequences.
High Amplification Efficiency
The PCR amplification efficiency was compared with other commercial Taq polymerase. LaboPass™ IP pro-Taq polymerase shows comparable or superior quality.
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PCR Performance
Various sizes of PCR products can be amplified using LaboPass™ IP pro-Taq DNA Polymerase.
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Long range PCR
IP pro-Taq Polymerase has proofreading activity and is more thermostable than wild type Taq DNA polymerase. Thus, IP pro-Taq is ideal for long range PCR.
Quality control
Each lot of IP pro-Taq polymerase, 10X IP pro-Taq buffer and dNTPs is tested for contamination such as E.coli genomic DNA, nicking, endo-nuclease and exo-nuclease.
| Test for nuclease activity Endonuclease and exonuclease activity were not detected after the incubation of 0.5 μg of supercoiled pUC19, λ DNA or HindIII digested λ DNA with 10 units of this enzyme for 4 hour at 37°C or 72°C. |
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Test for E.coli genomic DNA contamination
When compared with a competitor’s DNA polymerase, LaboPass™ IP pro-Taq Polymerase was verified to have no E.coli genomic DNA contamination.