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IP-Taq DNA polymerase
Product Description
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Brand | LaboPass |
| Category | DNA Polymerase | |
| Product Name | IP-Taq DNA Polymerase | |
| Storage Conditions | -20°C | |
| Download | Protocol  |
| Cat.No. | Product Description | Size (unit) | Price(JPY) |
| CMT1002 | LaboPass™ IP-Taq DNA Polymerase (2.5 u/μl) | 500 | 23,000 |
| CMT1005 | LaboPass™ IP-Taq DNA Polymerase (2.5 u/μl) | 2,500 | 97,000 |
| CMT1010 | LaboPass™ IP-Taq DNA Polymerase (2.5 u/μl) | 5,000 | 175,000 |
| CMT1302 | Mega3 LaboPass™ IP-Taq DNA Polymerase (2.5 u/μl) | 500×3 | 64,000 |
| CMT1305 | Mega3 LaboPass™ IP-Taq DNA Polymerase (2.5 u/μl) | 2,500×3 | 271,000 |
| CMT1310 | Mega3 LaboPass™ IP-Taq DNA Polymerase (2.5 u/μl) | 5,000×3 | 488,000 |
| CMT1502 | Mega5 LaboPass™ IP-Taq DNA Polymerase (2.5 u/μl) | 500×5 | 105,000 |
| CMT1505 | Mega5 LaboPass™ IP-Taq DNA Polymerase (2.5 u/μl) | 2,500×5 | 441,000 |
| CMT1510 | Mega5 LaboPass™ IP-Taq DNA Polymerase (2.5 u/μl) | 5,000×5 | 796,000 |
| CMT11002 | Mega10 LaboPass™ IP-Taq DNA Polymerase (2.5 u/μl) | 500×10 | 202,000 |
| CMT11005 | Mega10 LaboPass™ IP-Taq DNA Polymerase (2.5 u/μl) | 2,500×10 | 854,000 |
| CMT11010 | Mega10 LaboPass™ IP-Taq DNA Polymerase (2.5 u/μl) | 5,000×10 | 1,540,000 |
LaboPass™ IP-Taq DNA Polymerase is a thermostable DNA polymerase cloned from Thermus aquaticus and a recombinant form expressed in E.coli. This enzyme possesses 5′ to 3′ exonuclease activity, but lacks a 3′ to 5′ exonuclease proofreading activity. The enzyme purified with high purity contains a very low level of contaminating E.coli DNA, which minimizes false-positive results, especially when the amplicon is bacterial sequence (e.g. 16S rRNA).
Applications
-General PCR for detection
-Colony PCR
-Real-time PCR
-A-tailing for TA-cloning
Supplied Reagents
LaboPass™ IP-Taq DNA Polymerase is provided with an optimized buffer to improved PCR yield.
| 10X IP-Taq buffer I and II (with MgCl2) LaboPass™ IP-Taq DNA Polymerase is supplied with two types of reaction buffer with different salt formulation. Generally, Buffer I works well in most PCR reaction. The use of Buffer II can be tried if PCR products are not satisfactory (nonspecific, little or no products) using Buffer I. |
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5X Tuning buffer
Tuning buffer can improve PCR efficiency in reaction using problematic template DNA containing high GC contents or stable secondary structure. Thus, it is advantageous to amplify complicated long target sequences.
High Amplification Efficiency
The PCR amplification efficiency was compared with other commercial Taq polymerase. LaboPass™ IP-Taq polymerase shows comparable or superior quality.
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PCR Performance
Various sizes of PCR produces can be amplified using LaboPass™ IP-Taq DNA Polymerase.
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Quality Control
Each lot of IP-Taq polymerase, 10X IP-Taq buffer and dNTPs is tested for contamination such as E.coli genomic DNA, nicking, endo-nuclease and exo-nuclease.
| Test for nuclease activity Nicking, endonuclease and exonuclease activity were not detected after the incubation of 0.5 μg of supercoiled pUC19, λ DNA or HindIII digested λ DNA with 10 units of this enzyme for 4 hour at 37°C or 72°C. |
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Test for E.coli genomic DNA contamination
When compared with a competitor’s DNA polymerase, LaboPass™ IP-Taq polymerase verified to have no E.coli genomic DNA contamination.