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Yield & Domestic Order Lead time
- Yield Guarantee Non-modified DNA
- Yield Guarantee Modified DNA
- Domestic Order Lead Time : Non-Modified DNA
- Domestic Order Lead Time : Modified DNA
- Domestic Order Lead Time : Large Amount DNA Synthesis
Yield Guarantee Non-modified DNA
| Purification | Synthesis Scale | Available Length | Comments | ||||||
| -10 bases | 11-50 bases | 51-70 bases | 71-99 bases | 100-120 bases | 121-150 bases | 151-200 bases | |||
| Gel Filtration Reverse-phase cartridge purification |
OLIGOKIDS*6 | – | 1 OD | – | – | – | – | – | Only gel filtration and reverse-phase cartridge purification are available for OLIGO KIDS scale oligos with the length between 11 and 30 bases. |
| Gel Filteration*1 | 25nmol | – | 2 OD | – | – | – | – | – | Degenerated base*7 is available for more than 0.05µmol scale. |
| 0.05µmol | – | 5 OD | – | – | – | – | – | ||
| 0.2µmol | – | 8 OD | – | – | – | – | – | ||
| 1µmol | – | 30 OD | – | – | – | – | – | ||
| Reverse-phase cartridge purification*2
LC-ESI-MS-Reverse-phase cartridge purification*3 |
25nmol | – | 1 OD | – | – | – | – | – | Degenerated base*7 is available for more than 0.05µmol scale. |
| 0.05µmol | – | 3 OD | – | – | – | – | – | ||
| 0.2µmol | 1 OD | 5 OD | 5 OD | – | – | – | – | ||
| 1µmol | 5 OD | 20 OD | 20 OD | – | – | – | – | ||
| HPLC purification | 25nmol | – | 1 OD | – | – | – | – | – | Degenerated base*7 not available |
| 0.05µmol | – | 2 OD | – | – | – | – | – | ||
| 0.2µmol | 1 OD | 3 OD | 2 OD | 1 OD | – | – | – | ||
| 1µmol | 4 OD | 12 OD | 6 OD | 2 OD | 1 OD | – | – | ||
| IEX HPLC purification*4 | 0.2µmol | – | 1 OD | – | – | – | – | – | Available only in 0.2 µmol scale
Degenerated base*7 not available |
| High Purification*5 | 1µmol | – | 1 OD | – | – | – | – | – | Available only in 1µmol scale
Degenerated base*7 not available |
| PAGE Purification | 0.2µmol | – | 1 OD | 1 OD | 1 OD | – | – | – | Available only in 0.2µmol and 1µmol scale
Degenerated base*7 not available |
| 1µmol | – | 4 OD | 4 OD | 2 OD | 1 OD | 1 OD | 0.5 OD | ||
– Purification available for oligos less than 10 bases are reverse-phase cartridge, LC-ESI-MS-reverse-phase cartridge and HPLC.
Also, for oligos between 71 and 120 bases, HPLC or PAGE purification is available. For oligos between 121 and 200 bases, only PAGE purification is available
*1 Gel filtration is available for the oligos between 11 and 50 bases.
*2 Reverse-phase cartridge purification is available for the oligos up to 70 bases. HPLC purification or PAGE purification are recommended for the oligos longer than 71 bases.
HSS will not be responsible for the orders other than this specification.
*3 LC-ESI-MS data attachment is available only for the oligos up to 50 bases.
*4 IEX HPLC purification is available only for the oligos between 11 and 30 bases.
*5 High purification is available only for the oligos between 20 and 50 bases.
*6 More than two oligos order is required for OLIGO KIDS scale products. Only one oligo order is dealt as 25nmol scale product.
*7 HPLC and PAGE purifications are not available for oligos comprising degenerated base.
Please click here for detailed modification list.
Yield Guarantee Modified DNA
| Purification | Synthesis Scale | Available Length | Comments | |||||
| Up to 10 bases | 11-50 bases | 51-70 bases | 71-99 bases | 100-120 bases | 121-200 bases | |||
| Reverse-phase cartridge purification*1 | 0.2µmol | – | 1 OD | 1 OD | – | – | – | |
| 1µmol | – | 8 OD | 4 OD | – | – | – | ||
| HPLC Purification | 0.2µmol | – | 1 OD | 1 OD | 1 OD | – | – | Degenerated base not available |
| 1µmol | – | 8 OD | 4 OD | 2 OD | Pl. inquire | – | ||
– Orders for modified oligos are accepted in 0.2 µmol or more scale.
– Modified DNA is available only for HPLC purification except amination and phosphorylation which are available from Reverse-phase cartridge purification.
– Modification for 3′-end is available only for the oligos between 11 and 50 bases.
– For applying more than one modification, please inquire separately.
*1 Reverse-phase cartridge purification is available for the oligos up to 70 bases.
Please click here for detailed modification list.
Domestic Order Lead Time : Non-Modified DNA
| Purification | Available Length | -10 bases |
11-50 bases | 51-70 bases | 71-99 bases | 100-120 bases | 121-200 bases | Comments |
| Gel filtration | 11-50 bases | – | 1 | – | – | – | – | |
| Reverse-phase cartridge column purification | -70 bases |
2 | 1 | 2 | – | – | – | |
| LC-ESI-MS-Reverse-phase cartridge column purification*1 | -50 bases |
3 | 2 | – | – | – | – | LC-ESI-MS data attachment is available only for the oligos up to 50 bases. |
| HPLC purification | -120 bases | 3-4 | 2-3 | 3-4 | – | Degenerated base not available | ||
| IEX HPLC purification*2 | 11-30 bases | – | 3-4 | – | – | – | – | Available only in 0.2µmol scale Degenerated base not available |
| High purification*3 | 20-50 bases | – | 10 | – | – | – | – | Available only in 1µmol scale Degenerated base not available |
| PAGE purification | 11-200 bases | – | 6 | 7-12 | 9-14 | Available only in 0.2µmol or more scale Degenerated base not available |
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– Indicated lead time is the number of business days. (The order date is counted as “0” day.)
– For the orders of 1µmol or more scale, extra one day will be required to the above turnaround.
– Longer lead time may be required for more than 20 oligos orders as well as depending on sequence organization.
– HSS will not be responsible for the orders other than the available length in the above table.
– Purification available for oligos less than 10 bases are reverse-phase cartridge, LC-ESI-MS-reverse-phase cartridge and HPLC.
Also, for oligos between 71 and 120 bases, HPLC or PAGE purification is available, and for oligos between 121 and 200 bases, only PAGE is available.
*1 LC-ESI-MS-Reverse-phase cartridge purification is available for the oligos up to 50 bases.
*2 IEX HPLC purification is available only for the oligos between 11 and 30 bases.
*3 High purification is available only for the oligos between 20 and 50 bases.
Please click here for how to select the adequate purification.
Domestic Order Lead Time : Modified DNA
| Purification | Available Length | -10 bases |
11-50 bases | 51-70 bases | 71-99 bases | 100-120 bases | 121-200 bases | Comments |
| Reverse-phase cartridge column purification | 11-70 bases | – | 7-12 | 9-14 | Please inquire. | – | ||
| HPLC purification | 11-120 bases | Degenerated base not available | ||||||
– Indicated lead time is the number of business days. (The order date is counted as “0” day.)
– For the orders of 1µmol or more scale, extra one day will be required to the above turnaround.
– Longer lead time may be required for more than 20 oligos orders as well as depending on sequence organization.
– Modified DNA is available only for HPLC purification except amination and phosphorylation which are available from Reverse-phase cartridge purification.
– Modification for 3′-end is available only for the oligos between 11 and 50 bases.
– Delivery products can be dispensed. Please inquire.
Please refer to the Modification List for detail.
Domestic Order Lead Time : Large Amount DNA Synthesis
| Yield Guarantee | Non-Modified DNA | Modified DNA | Phosphorothioate DNA |
| 1-2mg | 7-12 | Please inquire. | 7-12 |
| 3-5mg | 7-12 | 7-12 | |
| 10mg | 9-14 | 9-14 |
– Indicated lead time is the number of business days. (The order date is counted as “0” day.)
– Purification available for miligram orders is only HPLC.
– Please inquire on the oligo more than 10mg.
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