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T4 DNA Ligase
Product Description
 |
Brand | LaboPass |
| Category | Modification Enzymes | |
| Product Name | T4 DNA Ligase | |
| Storage Conditions | -20℃ | |
| Download | Protocol  |
| Cat.No. | Product Description | Size(unit) | Price(JPY) |
| CMX0101 | LaboPass™ T4 DNA Ligase | 20,000 | 11,000 |
| CMX0105 | LaboPass™ T4 DNA Ligase | 100,000 | 45,000 |
LaboPass™ T4 DNA Ligase is isolated from a recombinant source. This enzyme catalyzes the formation of a phosphodiester bond between neighboring 5´ phosphate and 3´ hydroxyl termini of double-stranded DNA in either a cohesive or blunt-ended configuration. Single strand break in dsDNA is repaired by T4 DNA ligase. T4 DNA ligase can also catalyze the ligation of RNA to duplex DNA or RNA, but the activity toward single stranded DNA or RNA is very low.
| Applications – Cohesive or blunt termini ligation – Repair of nicks in duplex DNA or RNA – Linker or adaptor joining to DNA fragments |
Supplied reagents – T4 DNA Ligase – 10X T4 DNA Ligase buffer |
High ligation efficiency
1 μg of cohesive ended (λ DNA/HindIII), blunt ended (λ DNA/EcoRV) or linearized plasmid DNAs were incubated with each unit of T4 DNA Ligase in 1X Ligase Buffer for 30 min at 16°C. When compared with well-known brand, LaboPass™ T4 DNA Ligase shows comparable or better quality in ligation activity.
Very fast ligation
1 μg of λ DNA/HindIII DNA was incubated for indicated time with 400 unit of T4 DNA Ligase in 1X Ligase Buffer at 16°C. All DNA fragments is ligated in just 5 min.
