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cDNA Synthesis Kit
Product Description
 |
Brand | LaboPass |
| Category | RNA Related Products | |
| Product Name | cDNA Synthesis Kit | |
| Storage Conditions | -20℃ | |
| Download | Protocol  |
| Cat.No. | Product Description | Size(prep) | Price(JPY) |
| CMRTK001 | LaboPass™ cDNA Synthesis Kit | 50 | 37,000 |
| CMRTK002 | LaboPass™ cDNA Synthesis Kit | 100 | 72,700 |
LaboPass™ cDNA Synthesis Kit is optimized for efficient and reproducible synthesizing first-strand cDNA from total RNA or poly(A) RNA. This kit provides the all components necessary for synthesis of cDNA including reverse transcriptase, dNTPs, reaction buffer, RNase inhibitor, primer and nuclease-free water.
M-MuLV Reverse Transcriptase included in this kit is a recombinant form of the reverse transcriptase from the Moloney Murine Leukemia Virus (M-MuLV) which possesses enhanced cDNA synthesis activity and reduced RNase H activity. Reduction of RNase H activity enables higher yield of full-length cDNA transcripts and increased thermostability.
Applications
– cDNA synthesis for RT-PCR and RT-qPCR
– cDNA synthesis for gene cloning
| Supplied reagents | 50 rxn | 100 rxn |
| – Reverse transcriptase (200 U/μl) | 50 μl | 100 μl |
| – 5X RT buffer | 250 μl | 500 μl |
| – dNTP (each 10 mM) | 50 μl | 100 μl |
| – RNase inhibitor (40 U/μl) | 50 μl | 100 μl |
| – Oligo (dT)18 (100 μM) | 50 μl | 100 μl |
| – Random hexamer (0.2 μg/μl) | 50 μl | 100 μl |
| – Nuclease-free water | 1 ml | 1.5 ml |
High Yield of cDNA Synthesis
Efficiency of first strand cDNA synthesis was compared with other commercial enzyme. LaboPass™ M-MuLV Reverse Transcriptase shows comparable or superior quality.
Highly Sensitivity and Accuracy
First strand cDNA was generated from 1 ug to 100 pg of total HepG2 cell RNA with the LaboPass™ cDNA Synthesis Kit for RT-qPCR. Synthesized cDNA was used as a template in subsequent qPCR with LaboPass™ SYBR Green Q Master Mix on the Bio-rad CFX96 Real-Time PCR instrument. Parallel RT reactions demonstrate sensitive and reliable cDNA synthesis over a wide dynamic range of input RNA.
