Labozol Reagent

• SYBR Green Q Master

Product Description

	Brand	LaboPass
	Category	RNA Related Products
	Product Name	Labozol Reagent
	Storage Conditions	4℃　protected from light
	Download	Protocol

　

* Currency: JPY (tax exclusive)

Cat.No.	Product Description	Size	Price(JPY)
CMRZ001	LaboPass™ Labozol Reagent	100 ml	34,300

LaboPass™ Labozol Reagent  is a ready-to-use reagent, designed to isolate total RNA including miRNA from cell and tissue samples of human, animal, plant, yeast and bacterial origin. Labozol Reagent shows superior performance in extraction of total RNA with high integrity due to effective inhibition of RNase by phenol and guanidine thiocyanate, which are the major components in this reagent. The extracted RNA can be used in downstream applications such as cDNA synthesis, Northern blot, mRNA isolation, RNase protection assay and other RNA-based assay.

Applications
– mRNA isolation
– cDNA synthesis for RT-PCR, RT-qPCR and gene cloning
– Northern blot and RNase protection assay
– Other RNA-based experiments

Superior Performance in Total RNA Extraction

Total RNA was isolated from various cells and tissues with Labozol Reagent and run on denaturing agarose gel electrophoresis.

 

Expected Yields

The table below presents typical yields of RNA (A260/280 of >1.8) from various materials.

Material	Quantity	RNA (Yield)
Epithelial cells	1 X 106 cells	10 – 20 ug
Fibroblast cells	1 X 106 cells	7 – 8.5 ug
Brain	1 mg	1 – 1.4 ug
Spleen	1 mg	3 – 4.5 ug
Kidney	1 mg	4 – 6 ug
Liver	1 mg	5 – 6 ug

 

SYBR Green Q Master

Application to Real-Time Quantitative RT-PCR

Total RNA extracted from various cultured cells and tissues with Labozol Reagent was reverse-transcribed into cDNA using LaboPass™ cDNA synthesis kit (Cat.No. CMRTK001).
The resulting cDNA was applied to real-time qPCR for GAPDH mRNA using LaboPass™ SYBR green Q master (Cat.No. CMQS200).
Green : HEK293T   Blue : NIH3T3   Pink : mouse brain   Red : mouse liver   Orange : mouse kidney   Black : mouse spleen

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