Total RNA Kit

Product Description

Brand LaboPass
Category RNA Related Products
Product Name Total RNA Kit
Storage Conditions RT protected from light
Download Protocol

 

* Currency: JPY (tax exclusive)
Cat.No. Product Description Size(prep) Price(JPY)
CMRR0050 LaboPass™ Total RNA Kit 50 40,000
CMRR0100 LaboPass™ Total RNA Kit 100 70,000

LaboPass™ Total RNA Kit provides the simple and fast method for purification of total RNA from various biological samples, such as cultured cells and animal tissues. Highly pure RNA can be isolated in less than 30 minutes without any use of hazardous organic solvents and alcohol precipitation. Purified RNA can be directly used in various downstream applications without any further manipulations.

Applications
– cDNA synthesis
– PCR, RT-PCR, RT-qPCR
– Cloning

Superior Performance in Total RNA Extraction

Total RNA was isolated from various cells and tissues with Total RNA kit and run on denaturing agarose gel electrophoresis.

 

Expected Yields

The table below presents typical yields of RNA (A260/280 of >1.8) from various materials.

Material Quantity RNA (Yield)
Epithelial cells 1 X 106 cells 10 – 20 ug
Brain 1 mg 1 – 1.6 ug
Spleen 1 mg 2 – 3 ug
Kidney 1 mg 2 – 3 ug
Liver 1 mg 2 – 4 ug

Application to Real-Time Quantitative RT-PCR

Total RNA extracted with Total RNA kit can be successfully used in Real-time qRT-PCR assay.

Total RNA extracted from various cultured cells and tissues with Total RNA kit was reverse-transcribed into cDNA using LaboPass™cDNA synthesis kit (Cat.No. CMRTK001). The resulting cDNA was applied to real-time qPCR for GAPDH mRNA using LaboPass™ SYBR green Q master (Cat.No. CMQS200).

Back to "Products & Services"
Page Top