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IP-Pfu DNA Polymerase
Product Description
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Brand | LaboPass |
| Category | DNA Polymerase | |
| Product Name | IP-Pfu DNA Polymerase | |
| Storage Conditions | -20°C | |
| Download | Protocol  |
| Cat.No. | Product Description | Size (unit) | Price(JPY) |
| CMT4002 | LaboPass™ IP-Pfu DNA Polymerase (2.5 u/μl) | 500 | 88,000 |
LaboPass™ IP-Pfu DNA Polymerase is a thermostable DNA polymerase cloned from Pyrococcus furiosis and a recombinant form expressed in E.coli. This archaeal polymerase possesses 3’→5′ exonuclease proofreading activity as well as 5’→3′ polymerase activity, which allows high fidelity DNA amplification. Pfu polymerase retains its polymerase activity during extended exposure at 98°C unlike Taq polymerase. Therefore, this enzyme can be used to amplify difficult templates. (e.g. DNA with high GC content or stable secondary structure)
Applications
– High fidelity PCR
– Preparation of PCR products for cDNA cloning
– Site-directed mutagenesis
– Blunting of DNA ends
Supplied Reagents
LaboPass™ IP-Pfu DNA Polymerase is provided with an optimized buffer to improved PCR yield.
| 10X IP-Pfu buffer (with MgCl2 ) LaboPass™ IP Pfu DNA Polymerase is supplied with an optimized reaction buffer to improved PCR yield. |
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5X Tuning buffer
Tuning buffer can improve PCR efficiency in a reaction where template DNA contains high GC contents or stable secondary structures. Thus, it is advantageous to amplify complicated long target sequences.
High Amplification Efficiency
PCR amplification efficiency was compared with other commercial IP-Pfu polymerase. LaboPass™ IP-Pfu DNA polymerase shows comparable or superior quality.
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PCR Performance
Various sizes of PCR products can be amplified using LaboPass™ IP-Pfu DNA Polymerase.
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Quality Control
LaboPass™ IP-Pfu DNA Polymerase is represented by a very low error rate, determined based on β-galactosidase PCR mutation assay. Each lot of IP-Pfu polymerase, 10X Pfu buffer and dNTPs are tested for contamination such as E.coli genomic DNA, nicking, endo-nuclease and exo-nuclease.
| Test for nuclease activity Nicking, endonuclease and exonuclease activity were not detected after the incubation of 0.5 μg of supercoiled pUC19, λ DNA or HindIII digested λ DNA with 10 units of this enzyme for 4 hour at 37°C or 72°C. |
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Test for E.coli genomic DNA contamination
When compared with a competitor’s DNA polymerase, LaboPass™ IP-Pfu Polymerase was verified to have no E.coli genomic DNA contamination.