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PacBio Amplicon Sequencing
Overview
PacBio amplicon sequencing allows the determination of full-length sequences for PCR products approximately 1 – 2 kb in size.
Analysis Principle
PCR products are library-prepared without fragmentation, and a single molecule of cDNA is repeatedly sequenced within the small pores of the SMRT Cell.
The consensus sequence of reads from a single pore is referred to as the Circular Consensus Sequence (CCS). The CCS represents the full-length sequence information of the PCR product.
Workflow
Specifications
| Platform | Please inquire |
| Data Amount |
Data Analysis
【CCS Creation】
Creation of Circular Consensus Sequences (CCS) from sequencing data.
【Read Count】
Aggregation of identical sequences from the CCS, followed by counting the types and occurrences of detected sequences.