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GRAS-Di® Genotyping
What is GRAS-Di®?
GRAS-Di® is an advanced genotyping technology developed by Toyota Motor Corporation.
It leverages next-generation sequencing (NGS) to create a library of tens of thousands of amplicons using two rounds of PCR with high-concentration random primers and sequence adapter regions.
This innovative method supports genotyping not only with reference genomes but also with a proprietary algorithm that allows genotyping of organisms with unknown genomes or complex polyploidy.
* GRAS-Di® is a registered trademark of Toyota Motor Corporation.
* HSS offers licensed GRAS-Di® services under agreement with Toyota Motor Corporation.
Features of GRAS-Di®
・Exceptional reproducibility of amplicon amplification
・Minimal missing data in genotyping results
Supported Species (Over 60 Species Available)
| Rice | Radish | Apple | Cedar | |
| Wheat | Broccoli | Peach | Cherry | |
| Corn | Cauliflower | Pear | Human | |
| Sugarcane | Green onion | Orange | Cow | |
| Soybean | Onion | Citrus | Pig | |
| Peanut | Cucumber | Persimmon | Chicken | |
| Pea | Pumpkin | Grape | Horse | |
| Tomato | Spinach | Fig | Hamster | |
| Eggplant | Carrot | Kiwi | Guinea Pig | |
| Green Pepper | Burdock | Yuzu | Rat | |
| Bell Pepper | Strawberry | Sudachi | Tuna | |
| Potato | Melon | Prune | Shiitake Mushroom | |
* Other species are also supported. Please contact us for details.
GRAS-Di® Package Plan
Example Pricing (48 Samples)
RAW Data Delivery 700,000 JPY (excluding tax)
Included Services:
・ Quality check (conducted on 6 randomly selected samples)
・ GRAS-Di® library preparation
・ Sequencing using Illumina 150PE (1 lane, equivalent to 360 million read pairs)
Recommended Option
Pilot test (5 samples): 150,000 JPY (excluding tax)
・Trial library preparation with a small number of samples
・Compatibility test between your samples and random primers
Sample Requirements
Genomic DNA ≥ 50 ng
（Concentration: ≥ 2 ng/µL Volume: ≥ 20 µL）