Handling Instructions

• Product Storage
• Solution Preparation
• How to Read the Purification Report and Tube Label

Product Storage

Our synthetic DNA, RNA, and modified oligonucleotides are supplied in a dried form, except for certain products.
This is to prevent degradation caused by rapid temperature changes during transportation.

After arrival, the products can be kept in a dried state for several days without issue; however, we recommend storage at −20°C until use.

Once reconstituted with sterile water or buffer, repeated freeze–thaw cycles may increase the risk of degradation.
Therefore, we recommend aliquoting the solution into appropriate volumes for storage. Aliquoting also reduces the risk of contamination during use.

After aliquoting, store the products at −20°C.

Solution Preparation

Our synthetic DNA, RNA, and modified oligonucleotides appear as a transparent film or white crystals when dried.
As the product may be difficult to see, please centrifuge briefly before opening the tube.

By adding the volume indicated under “TE Volume” on the purification report, the product can be reconstituted to a concentration of 100 pmol/µL.
Please refer to this value when preparing the solution.

How to Read the Purification Report and Tube Label

Important information required for using the product is provided in the purification report and on the tube label. Details of each item listed in the purification report and on the tube label are shown below.

By adding the volume indicated under “TE Volume (TE)”, the product can be reconstituted to a concentration of 100 pmol/µL.

Purification Report	Tube Label	Description
精製方法	ｹﾞ、脱、簡 H、ｲ、P、高	The purification method specified by the customer. ｹﾞ = Gel filtration、脱 = Desalting、簡 = Cartridge、H = HPLC、ｲ = Ion-exchange HPLC、P = PAGE、高 = High purity
塩基数		Indicates the length of the synthetic oligonucleotide (mer).
分子量	MW	Theoretical molecular weight calculated by our company. MS results are evaluated by comparison with this value.
ABS		Measured absorbance value. * Absorbance of a solution prepared by diluting 1 mL of the reconstituted product 50-fold (or a value calculated from the dilution factor).
OD値	OD	The guaranteed yield is based on the value shown here.
全量	TW	Total weight of product expressed in µg. * The guaranteed amount is defined by the OD value above.
全mol数	モル	The total weight divided by the theoretical molecular weight (Unit: nmol).
TE量	TE	The volume of solution required to obtain a concentration of 100 pmol/µL, based on the total moles (Unit: µL).
Tm値	TM	Temperature at which 50% of the duplex becomes single-stranded upon thermal denaturation. * Values for modifications are not included in the Tm calculation.

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