Agilent Gene Expression Microarray Analysis

• Description
• Price and Domestic Lead-Time
• Required Sample
• Ordering
• Cautions

Description

Features

• – Uses Agilent microarrays, highly rated for sensitivity and precision
• – All processes, from sample quality inspection to data analysis, are performed in-house
• – As an official certified service provider of Agilent, we obtain reliable data
• – Offers cost-effective shared plans for small-scale human and mouse experiments

 

Service Contents

01　Sample QC	02　Microarray Experiment	03　Basic Analysis
We evaluate the sample quality using NanoDrop, Bioanalyzer, or TapeStation. Basic charge covers up to twice of sample QC.	Entire microarray experiments such as fluorescent dye labeling of nucleic acid samples, hybridization to array slides and obtaining raw data are acceptable.	We normalize the data and extract differentially expressed genes by comparing samples or groups. The normalization method and thresholds are adjusted according to each data.

 

Analysis Plan

We offer the following two analysis plans for customers ordering to analyze human (SurePrint G3 Human GE 8x60K Ver.3.0) or mouse (SurePrint G3 Mouse GE 8x60K Ver. 2.0). For other arrays, only the standard plan is available.

	Analysis is performed on per-slide. Analysis begins once the sample QC is passed and the array is in stock. Ideal for customers who need fast results.
	For customers who want to analyze fewer than 8 samples. By sharing 8 arrays on a slide with other customers, analysis can be done at a reduced cost. Recommended for customers who want to analyze a small number of samples cost-effectively.

Deliverables

Item	Contents	Format	Details
Document	Analysis Report	－	－
Recording Media – CD-R – DVD-R – USB	Raw data	txt	Signal data output from microarray system
	Microarray scanned image	tif, bmp	Images obtained by scanner, from which raw data is obtained
	Signal table	xlsx	Signal intensity(Raw, Normalized, Baseline transformed), LogFC and list of differentially expressed genes in sample comparison
	Visual summary	html	Box plot, Scatter plot, Correlation, Principal component analysis, Clustering, Sample information
	Reference guide	pdf	Reference guide for raw data and analyzed data
	Protocol	pdf	Protocol for microarray experiments, condition value of the works from experiments to data analysis
	Sample QC report	pdf	Data of sample quality control
	Analysis Report	pdf	PDF data of the analysis report document

• * Our data storing period is 6 months after delivery. Please keep the data in a safe place as we may ask you to provide the data in case of inquiry or additional analysis request after the storing period has been expired.

 

Price and Domestic Lead-Time

Service		Domestic Lead-time	Price
Agilent Gene Expression Microarray Analysis	Shared plan – 1color	3 weeks (up to 3 months) after meeting the below requirements; – Sample QC passed – Number of samples required for the share plan	Open price
	Shared plan – 2color
	Normal Plan – 1color	3 weeks after meeting the below requirements; – Analysis slide delivered – Sample QC passed
	Normal Plan – 2color

• * Sample QC fee (twice incl. sample reshipment) is included in the price.
• * The lead-time may vary according to each order specifications such as the number of analyses.
• * Shared plan is only available for analyses with “SurePrint G3 Human 8x60K v3” or “SurePrint G3 Mouse 8x60K v2“.

 

Required Sample

Sample Type	Required Amount	Conditions
Total RNA	Total amount:500ng or more Concentration:50ng/μL or more Volume: 10uL or more	A260/A280：1.8 – 2.0 A260/A230：2.0 or more Dissolved in Nuclease Free Water

• * We recommend using QIAGEN RNeasy Mini Kit for RNA extraction and RNase-Free DNase Set for DNase treatment.
• * DEPC water may inhibit reactions. Please make sure to dissolve RNA to Nuclease Free water.

 

Ordering

Order Flow

How to ship your sample

RNA sample
To prevent a sample tube from breakage, please put it in another container such as 50mL tube or tube box and pack it together with a printed order sheet and approx. 5kg of dry ice to dispatch under refrigerated or frozen state.

• * For any sample shipping, please let us know the below information by e-mail.
  1. delivery schedule 2. courier 3. tracking number.
• * Please make sure that your package will be delivered to us on working days.
• * Remnants of samples will be stored for 3 months, then be discarded.If sample return is required, please describe so on your order sheet at the order placement.
• * Please note that samples are returned free of shipping charge only when they are returned with data delivery.

 

Cautions

• – Description and scope of the service are subject to changes without notice.
• – Cancellation of an order will be rejected after production start. If cancellation is due to force majeure, the client will be asked to pay the service up to the cancellation point.
• – Samples are limited to the P1 level defined in “the Law concerning the Conservation and Sustainable Use of Biological Diversity through Regulations on the Use of Living Modified Organisms” (Cartagena Protocol) issued by Ministry of Education, Culture, Sports, Science and Technology.
• – Infective samples such as HCV or HIV are not acceptable. In case of using human clinical sample, please confirm if the informed consent is obtained.
• – The result of this custom analysis service should be used for the purpose of investigation.The result should not be used for purposes such as production/quality control of a medical product or food, diagnosis, etc.
• – HSS will not be responsible for losses a client may incur as a result of the custom analysis service output.
• – HSS will not be responsible for troubles related to industrial property rights or safety whose origin is in the sample submitted by a client or the methods used.

 

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