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LipoTrust™ EX Series Data Page
LipoTrust™ EX
LipoTrust™ EX Oligo
Transfection reagent for short oligonucleotides (e.g. siRNA,antisense DNA,miRNA etc.)
LipoTrust™ EX Oligo
Protocol download: in vitro
MSDS download: in vitro
Data1 : Knock-down efficiency – comparison with competitive products
Data2 : Cytotoxicity with trasfection visualized by photo
Data3 : FCM (flow cytometry) data
Data4 : Confocal laser scanning microscope photos
Data5 : Wide ranging use
Data6 : Transfection experiment flow
Data7 : Successful examples of cell transfection
Data1：Knock-down efficiency – comparison with competitive products
This graph shows knock-down efficiency in the experiment of 40nM anti-Luciferase siRNA transfection into HeLa cell which carries Luciferase genes. The height of bars shows knock-down efficiency, and 100% means that Luciferase activities are completely knocked down to zero. Loading quantity of each transfection reagent is according to respective protocols.
Data2：Cytotoxicity with transfection
* Status of cells right before the Luciferase activities measurement using competitive products and LipoTrust™ EX Oligo at the above experiment.
Data3：FCM (flow cytometry) data
FCM (flow cytometry) analysis was performed to each Caco-2 cell 48 hours after the transfection of scrambled siRNA labelled with FITC by either each of competitive products or LipoTrust™ EX Oligo.Orange lines show those of cell which no transfection was performed respectively.
Data4：Confocal laser scanning microscope photos
Photos taken 24 hours after the transfection of scrambled siRNA labelled with FITC into HeLa cell. Successfully transfected siRNA were effectively diffused inside the cells.
Data5：Wide ranging use
Transfection experiments with HeLa cell which carries Luciferase genes were performed in 96 well plate. Concentrations of both anti-Luciferase siRNA and LipoTrust™ EX Oligo were varied as far as no cytotoxicity was shown. The height of bars means Luciferase activity level, and 100% value represents Luciferase activity obtained from non-transfected cell.
Data6：Transfection experiment flow (The figure shows only the image of experiment.)
Data7：Successful examples of cell transfection
|
Cell type
|
Transfection Efficiency Level
|
| HeLa Cell | Excellent |
| CHO Cell | Excellent |
| KB Cell | Excellent |
| HT1080 Cell | Good |
| 3T3-L1 Cell | Good |
| COS-1 Cell | Excellent |
| Caco-2 Cell | Good |
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LipoTrust™ EX Gene
Transfection reagent for plasmid DNA
LipoTrust™ EX Gene
Protocol download
MSDS download
Data1： Products Comparison (HeLa cell)
Data2： Products Comparison (Reagent I) for various cells
Data3： Successful examples of Cell transfection
Data1：Products Comparison (HeLa cell)
– LipoTrust™ EX Gene shows more transfection efficiency than the other reagents (Reagent I ~ VI).
Data2：Products Comparison (Reagent I) for various cells
– LipoTrust™ EX Gene marks high transfection efficiency also in the various cells other than HeLa, such as 293, G2, 313-L1, CHOK1 and HT1080.
Plasmid DNA (β-galactosidase gene) were transfected into each cell respectively.
β-galactosidase activities were measured 24 hours after transfection.
* The results are shown as comparison value The activity of LipoTrust™ EX Gene is shown as 1.0.
Data3：Successful examples of cell transfection
|
Cell type
|
Transfection Efficiency Level
|
| HeLa Cell | Excellent |
| HT1080 Cell | Excellent |
| NB1RGB Cell | Good |
| 293T Cell | Excellent |
| COS-1 Cell | Excellent |
LipoTrust™
Protocol download
MSDS download: SR , PE , CH
Data1： Structure of DC-6-14, the principal component of LipoTrust™
Data2： Electronography of the former liposomes in the presence of serum
Data3： Highly efficient gene expression of LipoTrust™ SR in the presence of Serum (1)
Data4： Highly efficient gene expression of LipoTrust™ SR in the presence of Serum (2)
Data5： Characteristics of each liposome of LipoTrust™ Set in the absence of Serum
Data6： Highly efficient gene expression of LipoTrust™ SR in in vivo experiment
Data7： Successful examples of cell transfection
Data1： Structure of DC-6-14, the principal component of LipoTrust™
O,O’-ditetradecanoyl-N-(α-trimethylammonioacetyl)diethanolamine chloride (DC-6-14)
Data2：Electronography of the former liposomes in the presence of serum
These pictures show change of liposome form when they are disposed in the medium containing 5% FBS. The nanoparticle structures disappeared in the medium with 15min in the case of Other product, while spherical forms are still retained after one hour with LipoTrust SR.
Data3：Highly efficient gene expression of LipoTrust™ SR in the presence of Serum (1)
This figure shows Luciferase activity after the transfection of Luciferase gene into HRA cells (Human ovarian tumor cells) using either LipoTrust™ SR or one of several other commercial products in 10% FBS-containing medium. LipoTrust™ SR shows remarkably higher efficient gene expression than the other products.
Data4：Highly efficient gene expression of LipoTrust™ SR in the presence of Serum (2)
This figure shows the level of LacZ-positive cells after the transfection of LacZ gene into several kinds of human tumor cells by either LipoTrust™ SR or one of several other commercial products in 10% FBS-containing medium. LipoTrust™ SR shows remarkably higher efficient gene expression than the other products.
Data5：Characteristics of each liposome of LipoTrust™ Set in the absence of Serum
This figure shows Luciferase activity after the transfection of Luciferase gene into human tumor cells using LipoTrust™ set in serum-free medium.
LipoTrust™ CH shows highly efficient gene expression in serum-free medium.
Data6：Highly efficient gene expression of LipoTrust™ SR in in vivo experiment
| Type of Liposomes |
Relative expression of transfected cells
|
|
LipoTrust™ SR
|
1.00 ± 0.11
|
|
Product A
|
0.38 ± 0.26 |
|
Product B
|
0.62 ± 0.21
|
|
Product C
|
0.23 ± 0.23 |
5 x 106 mEIIL cells were seeded in abdominal cavity of nude mouse. After three weeks, 1ml of liposome / CAG-LacZ gene (20µg) was administered to the abdominal cavity of the mouse, and on the following day, cells were picked from the abdominal cavity. This table shows the rate of LacZ-positive cells of the picked cells. LipoTrust™ SR shows highly efficient gene expression than the other commercial products.
Data7：Successful examples of cell transfection
|
Cell type
|
Transfection Efficiency
|
Cell type | Transfection Efficiency |
| 293 | Excellent | HRA | Excellent |
| 3T3 | Good | Jurkat | Good |
| A431 | Good | MDCK | Good |
| A549 | Good | ｍEIIL | Excellent |
| B95a | Good | Morris | Excellent |
| C33A | Good | Neuro-2A | Excellent |
| COS-1 | Good | PC3 | Good |
| COS-7 | Excellent | RBL-2H3 | Excellent |
| ES | Excellent | Walker-256 | Excellent |
| H322 | Good | Mouse Fibroblast | Excellent |
| H520 | Good | Mouse Granule Cell (Neuron) | Excellent |
| HeLa | Excellent | Calf Vascular Endothelial Cell | Good |
| HepG2 | Good | Dorsal Root Ganglion | Excellent |
Reference
Rai K,Takigawa N, Ito S, Kashihara H, Ichihara E, Yasuda T et al.
Liposomal Delivery of MicroRNA-7-Expressing Plasmid Overcomes Epidermal Growth Factor Receptor Tyrosine Kinase Inhibitor-Resistance in Lung Cancer
Cells. Mol Cancer Ther. 2011;10(9):1720-7
Efficient delivery of liposome-mediated MGMT-siRNA reinforces the cytotoxity of temozolomide in GBM-initiating cells T Kato, A Natsume et al.
Gene Therapy, 1-9 (2010)
Knocking down barriers:advances in siRNA deliveryKathryn A. Whitehead, Robert Langer and Daniel G. Anderson Nature Review siRNA delivery 129-138
Poster #1396: MicroRNA-7 downregulates epidermal growth factor receptor in both gefitinib-sensitive and resistant lung carcinoma cells: presented by Kammei Rai et al 100th AACR in April 19, 2009.
Resolution of liver cirrhosis using vitamin A-coupled lipos Development of novel cationic liposomes for efficient gene transfer into peritoneal disseminated tumor.
Hum. Gene Ther.,10,947-955 (1999)
Development of novel cationic liposomes for efficient gene transfer into peritoneal disseminated tumor.
Hum. Gene Ther.,10,947-955 (1999)
Gene delivery using liposome technology.
J. Control.Release, 62,269-277(1999)
A new cationic liposome for efficent gene delivery with serum into cultured human cells: A quantitative analysis using two independent fluorescent probes.
Biochim.Biophys.Acta,1467,419-430(2000)
Characteristics and biodistribution of cationic liposomes and their complexes.
J.Control.Release,69,139-148(2000)
Phorbol ester-potentiated liposomal transfection to monocytic PLB-985 cells.
J.Biochem.,128,989-998(2000)
Quantative analysis of correlation between number of nuclear plasmids and gene expression activity after transfection with cationic liposomes.
Pharm.Res.,19,377-381(2002)
PU.1 is dominant and HAF-1 supplementary for activation of the gp91phox promoter in human monocytic PLB-985 cells.
J.Biochem.,131.533-540(2002)
Norepinephrine enhances fibrosis mediated by TGF-
in cardiac fibroblast.
Hypertension,40,148-154(2002)
Lipoeomes base on nanotecnology: Past, present and future (Part II).
PHARM TECH JAPAN,19,419-433(2003)
Application of stealth-liposomes to anti-cancer chemotherapy and gene therapy.
Biotherapy,18,353-360(2004)
Ultrasound enhances liposome-mediated gene transfection.
Ultrasound Sonochemistry,Accepted.