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Total RNA Kit
Product Description
 |
Brand | LaboPass |
| Category | RNA Related Products | |
| Product Name | Total RNA Kit | |
| Storage Conditions | RT protected from light | |
| Download | Protocol  |
| Cat.No. | Product Description | Size(prep) | Price(JPY) |
| CMRR0050 | LaboPass™ Total RNA Kit | 50 | 40,000 |
| CMRR0100 | LaboPass™ Total RNA Kit | 100 | 70,000 |
LaboPass™ Total RNA Kit provides the simple and fast method for purification of total RNA from various biological samples, such as cultured cells and animal tissues. Highly pure RNA can be isolated in less than 30 minutes without any use of hazardous organic solvents and alcohol precipitation. Purified RNA can be directly used in various downstream applications without any further manipulations.
Applications
– cDNA synthesis
– PCR, RT-PCR, RT-qPCR
– Cloning
Superior Performance in Total RNA Extraction
Total RNA was isolated from various cells and tissues with Total RNA kit and run on denaturing agarose gel electrophoresis.
Expected Yields
The table below presents typical yields of RNA (A260/280 of >1.8) from various materials.
| Material | Quantity | RNA (Yield) |
| Epithelial cells | 1 X 106 cells | 10 – 20 ug |
| Brain | 1 mg | 1 – 1.6 ug |
| Spleen | 1 mg | 2 – 3 ug |
| Kidney | 1 mg | 2 – 3 ug |
| Liver | 1 mg | 2 – 4 ug |
Application to Real-Time Quantitative RT-PCR
Total RNA extracted from various cultured cells and tissues with Total RNA kit was reverse-transcribed into cDNA using LaboPass™cDNA synthesis kit (Cat.No. CMRTK001). The resulting cDNA was applied to real-time qPCR for GAPDH mRNA using LaboPass™ SYBR green Q master (Cat.No. CMQS200).
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