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Universal Primer List
The following universal primers are available free of charge with OnePass or Full Service for DNA Sequence Analysis or with Primer Walking Service. If you wish to use any of these primers, please specify your choice on the order form. Due to character limitations, please use the abbreviation.
| Primer Name | Abbreviation | Sequence |
|---|---|---|
| T7 promoter | T7pro | TAA TAC GAC TCA CTA TAG G |
| T7 terminator | T7term | GCT AGT TAT TGC TCA GCG G |
| T3 | T3 | AAT TAA CCC TCA CTA AAG GG |
| M13FW | M13FW | GTA AAA CGA CGG CCA GT |
| M13RV | M13RV | GGA AAC AGC TAT GAC CAT G |
| M13 P1 | M13P1 | GTA AAA CGA CGG CCA GT |
| M13 P5 | M13P5 | CAG GAA ACA GCT ATG AC |
| M13 M4 | M13M4 | GTT TTC CCA GTC ACG AC |
| SP6 | SP6 | TAT TTA GGT GAC ACT ATA G |
| pGEX5′ | pGEX5 | GGG CTG GCA AGC CAC GTT TGG TG |
| pGEX3′ | pGEX3 | CCG GGA GCT GCA TGT GTC AGA GG |
| BGH-Reverse | BGH-R | TAG AAG GCA CAG TCG AGG |