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Nucleic Acid Extraction

Inquiry

　* Samples containing Covid-19 or influenza viruses are not to be accepted.

As we currently produce oligos to be used in PCR test kits for [SARS-CoV-2] and [Influenza A/B virus], we do not accept nucleic acid samples which may become a template for such oligos. Thank you for your understanding.

 

Service Description

We will extract and purify gDNA or total RNA from your provided samples.
Extracted and purified nucleic acid is subject to QC by TapeStation or Bioanalyzer.

 

Samples

Tissue, cell, blood and the like from Human or animals

Please inquire in advance for below cases;
– Any fibrous or rigid tissue which is hard to be fractured
– Non Human / animal samples (e.g. plant, fungus, bacteria, environmental samples)
– Extraction from a section
– Extraction of total RNA which contains small RNA
* For Human clinical sample orders, please make sure to confirm the “About human clinical samples” section described below.

 

Required Amount of Sample

DNA Extraction
Sample Type Approx. Required Amount How to prepare Container for transport
Tissue square 3 mm on a side (approx. 30mg) Freeze a tissue fragment instantly by using liquid nitrogen and store it at -80 degree C 1.5 or 2mL tube
Cell approx. 106 cells Freeze a cell pellet instantly by using liquid nitrogen and store it at -80 degree C 1.5 or 2mL tube
Blood 500uL or more Collect blood using blood collection tube with anticoagulant agent, split the required amount and freeze it at -80 degree C 1.5 or 2mL tube
RNA Extraction
Sample Type Approx. Required Amount How to prepare Container for transport
Tissue square 3~5 mm on a side (approx. 30~100mg) After RNAlater treatment*1 or freezing with liquid nitrogen instantly, store the tissue fragment at -80 degree C 1.5 or 2mL tube
Cell approx. 106 cells Freeze a cell pellet using liquid nitrogen instantly and store it at -80 degree C 1.5 or 2mL tube
Blood 2.5mL Collect blood in PAXgene® Blood RNA Tube (2.5mL)*2, leave it at room temperature for at least 2 hours, and freeze it at -20 degree C for at least 24 hours PAXgene®Bolld RNA Tube
*1 Please provide us a tissue with the thickness less than 5mm. For thicker tissue fragments, RNA in the center part becomes unstable due to the reagents penetration delay, which may cause RNA degradation. Please note that RNA later treatment is not available for frozen tissues.
*2 Please use PAXgene® RNA blood collection tube (Becton, Dickinson and Company, Cat. no. 762165) to collect Human blood for use of RNA extraction. Please refer to the web site of Becton, Dickinson and Company for how to use the tube.

 

Price

The extraction service fee is same for both DNA and RNA.
The service is available for nucleic acid extraction only as well as for the set with NGS or microarray analysis service.

(Tax exclusive)
Number of Sample Only for nucleic acid extraction service Set price with analysis such as microarray and NGS
Unit Price Total Price Unit Price Total Price
1 46,000 JPY / sample 46,000 JPY 40,000 JPY / sample 40,000 JPY
2 34,000 JPY / sample 68,000 JPY 28,000 JPY / sample 56,000 JPY
3 31,000 JPY / sample 93,000 JPY 25,000 JPY / sample 75,000 JPY
4 26,000 JPY / sample 104,000 JPY 20,000 JPY / sample 80,000 JPY
5 23,000 JPY / sample 115,000 JPY 17,000 JPY / sample 85,000 JPY
6 21,000 JPY / sample 126,000 JPY 15,000 JPY / sample 90,000 JPY
* The above price is for 1 prep.
* Price may be differed depend on the amount or status of samples to be provided.

 

Ordering

For Human clinical samples, please make sure to read through the“Cautions about human clinical samples”  described below .
The way of order is different between Human clinical samples and other samples.

Please inquire before shipping your samples.
Upon discussion with our staff, please send the sample(s) together with the order sheet to the below address;

　◆Please send your samples to:
Attn. Analysis Team,
Hokkaido System Science Co., Ltd.
2-1, Shinkawa Nishi 2-1, Kita-ku, Sapporo, 001-0932
TEL: (011)768-5903
E-mail：bio@hssnet.co.jp

* Please clearly state as “sample for nucleic acid extraction service”.
* Please make sure to enclose enough amount of dry ice so that the sample is kept frozen until it is delivered to us.
* We do not receive samples on Saturdays, Sundays and national holidays. Please make sure that your package will be delivered to HSS on working days from 9:00 to 18:00.
* HSS is not responsible for any troubles during the transport.

 

Cautions about Human Clinical Samples

Below samples are accepted as Human clinical samples;

– Samples other than “generally available samples such as cell lines”
– Samples derived from any specific patient

Regarding the orders for Human clinical samples, please make sure to confirm the below descriptions.

Sample Acceptability

We basically do not accept samples infected with pathogenetic microorganism or virus.
Please refer to the below for details.

× We do not accept below samples;
1. Samples infected with microorganisms whose pathogenetic level to Human is bio safety level (BSL) 3 or higher according to “safety management code for handling pathogenic microorganisms” published by the National Institute of Infectious Diseases
2. Samples which are not determined to have a low possibility of infection with the microorganisms mentioned in the above section 1 based on clinical tests and findings
3. Samples infected with HCV or HBV virus
4. Samples infected with other pathogenetic microorganisms or virus whose bio safety level are not determined as BLS 2 or less.
* Please make sure to obtain the informed consent for such samples.

 

How to order

Sample Information Required

– It is required to provide sample information in advance so that HSS confirms if the sample is acceptable.For details, please inquire.

Sample Shipping

– Please ship out only the samples which are confirmed as acceptable by HSS.

– Please ship your sample using our Human sample shipping kit.
This kit will be sent from HSS upon confirmation of sample acceptability based on the information provided by a client.
Shipping kit and the way of packing differ depend on extracted nucleic acid and the sample type.
For details, please inquire.

CONTACT

Inquiry

If you have any questions about our services, please do not hesitate to inquire.

Tel: 011-768-5901 

(9:00-17:00, except for Sat., Sun. and national holidays)

 

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