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Small scale sequencing (NGS Petit)
Overview
This service offers DNA sequencing of viral genomes, plasmids, long-PCR products, and other DNA molecules ranging from several kilobases to approximately 200 kb.
Using the Illumina MiSeq platform, we provide rapid, cost-effective sequencing with data acquisition starting from approximately 60 Mb through shared MiSeq runs.
Using the Illumina MiSeq platform, we provide rapid, cost-effective sequencing with data acquisition starting from approximately 60 Mb through shared MiSeq runs.
Applications
・ Complete genome sequencing of viral DNA
・ Full-length sequencing of plasmid DNA
・ Full-length sequencing of long-PCR products
<Additional Applications>
・ Microsatellite region detection in genomic DNA of higher organisms (low-coverage analysis)
・ Amplicon-Seq analysis of PCR products (200 – 450 bp) without linker sequences or fragmentation
Specifications
| Platform | Illumina MiSeq |
| Library Preparation | PCR-Plus / PCR-Free (optional) |
| Read Configuration | Paired-End (300 bp x2) |
| Data Amount | 60 Mb (100,000 read pairs)*1 |
| Data Analysis | Please inquire.*2 |
*1 Data Amount is adjustable in 60 Mb units. Contact us in advance for custom requests.
*2 Data analysis services, including assembly, mapping, or custom workflows, are available based on your research needs.
Sample Requirements
| Sample Type | Total Amount | Concentration | Volume |
| DNA | ≥ 4 µg | ≥ 40 ng/µL | ≥ 30 µL |
| Purified Long-PCR Products | ≥ 4 µg | ≥ 40 ng/µL | ≥ 30 µL |
| Purified PCR Products (~800 bp) | ~ 2 µg | ~ 20 ng/µL | ≥ 30 µL |