Support & Inquiry Ordering JP

Capillary Sequencing

Ordering Inquiry

* Samples containing Covid-19 or influenza viruses are not to be accepted.
As we currently produce oligos to be used in PCR test kits for [SARS-CoV-2] and [Influenza A/B virus], we do not accept nucleic acid samples which may become a template for such oligos. Thank you for your understanding.

 

We provide high-quality DNA sequencing using the Sanger method, offering rapid and cost-effective solutions. Our skilled staff ensures reliable data and can handle difficult sequences by optimizing conditions. Our diverse services accelerate your research and development.

DNA Sequencing

Send us your template DNA and primers, and we will provide you with sequencing data.

  • Pre-mix
  • One-pass
  • Full
Long Sequence Analysis

Analyze long DNA sequences step-by-step using primer walking.

  • Primer Walking
Optional Services

Additional services related to pre-sequencing processing.

  • DNA Extraction
  • Primer Design/Synthesis
  • PCR Amplification
  • PCR Product Purification

 

DNA Sequencing

We sequence the DNA samples you send us.
Perform a single cycle sequencing reaction, and deliver the data.

Work Process
Sequencing Reaction
Sequencer Run
Data Delivery
Reagents
BigDye Terminator v3.1
Sequencer
3730xl DNA Analyzer
Average sequencing length
approx. 500 – 700 bases
(depending on sample quality)

Analysis Plan

Pre-mix
One-pass
Full
✓ Low-cost ✓ No sample preparation required ✓ Sample QC and
re-analysis guarantee
One-off service
for template and primer
mixing samples.		 One-off service
for templates and primers
to be sent separately.		 Full service
including sample QC
and re-sequencing.
Price（1 tube）
Order Unit: 1 seq
 1,500 JPY / 1 tube 2,000 JPY / 1 tube 3,500 JPY / 1 tube
Sample QC
 –
Only available for orders of 1 tube.
Sample preparation
 –
Universal primers available.
Universal primers available.
Sequencing
Run Time：1hr
Run Time：1hr
Run Time：2hr
Re-analysis
 – –
Discounted Pricing for Multiple Samples
8-Tube Strip
Order Unit: 8 seq
 5,600 JPY / 8-Tube Strip
700 JPY/analysis		 12,000 JPY / 8-Tube Strip
1,500 JPY/analysis		 Full-service orders are available only for single-tube analyses.
0.5 Plate
Order Unit: 48 seq
 33,600 JPY / 0.5 Plate
700 JPY/analysis		 43,200 JPY / 0.5 Plate
900 JPY/analysis
1 Plate
(1-9 Plates)
Order Unit: 96 seq
 44,160 JPY / 1 Plate
460 JPY/analysis		 52,800 JPY / 1 Plate
550 JPY/analysis
1 Plate
(10+ Plates)
Order Unit: 96 seq
 41,280 JPY / 1 Plate
430 JPY/analysis		 48,000 JPY / 1 Plate
500 JPY/analysis
  • * Prices are exclusive of tax.
  • * Prices listed are for single-sequence (single-strand) analysis.
  • * If the order quantity is below the specified unit, the full unit price will be charged.
  • – Pre-mix, One-pass 8-strip, and One-pass plate do not include sample QC. The samples are used as provided, which may affect results if concentrations are not met.
  • – For difficult sequences like GC rich or high-order structures, please note this in the order form.
  • – Re-sequencing for poor data quality due to other causes will be treated as a new order. Please note that no investigation of causes is included in the service.

Turnaround Times

Single Tube
2 business days
8-Tube Strip
3 business days
Plate
4 + business days
  • * Turnaround time is calculated from the day the sample arrives as “Day 0”. Please note that the delivery time calculation is based on business days and does not include weekends and public holidays.
  • * Same-day sample receipt is available until 3:00 PM. Samples received after 3:00 PM will be processed the next business day.
  • * Turnaround times may vary depending on the order details and workload. Additionally, re-sequencing may require additional time. Please be aware of these factors.

Delivery

Results are provided via web download. A URL will be emailed when data is ready.

Delivered Data
・Sequence Data (.txt)
・Raw Data (.ab1)
  • * Data is deleted from the server 3 months after delivery.
For New Users, we inform free softwares for viewing Ab1 files.

Required for Analysis

Pre-mix

DNA Template and Sequencing Primer
mix sample

 Plasmid (450 – 900ng) or PCR product (appropriate amount for amplicon size) and Sequencing Primer (10pmol) mixed in 20μL sterile water

Order Form

 Download the order form for your desired analysis unit and fill it out.
One-pass
Full

DNA Template

 Plasmid (Conc. 100 – 300ng/μL, 20+ μL)
or PCR product (Conc. 10 – 50ng/μL, 20+ μL)

Sequencing Primer

 Conc. 3.2μM (10+ μL)
Universal primers are available on request.

Order Form

 Download the order form for your desired analysis unit and fill it out.

Primer Usage for One-pass 8-Strip / Plate

  • – Up to 2 primers per 8-strip or plate. For 3+ primers, prepare a primer plate aligned with sample layout.
  • – For 3+ primers, prepare a primer plate aligned with sample layout. For detailed instructions on preparing primer plates, please refer to Preparation of Primer Plates.

Sample Preparation Guide　Order Procedure　

Additional Analysis

If you wish to perform additional analysis on the same sample using the One-Pass or Full Service, it is possible to reanalyze the sample you have already sent.

Order Process

  • ●Confirm sample amount for additional analysis.
  • ●Download and complete the appropriate order form from our website. Create a new order form and mark “Additional Analysis” in the notes.
  • ●Email the form to seq@hssnet.co.jp

Order Period

Samples are stored for 3 business days after analysis completion.
Order within this period for additional analysis.

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