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Analysis of N-terminal Amino Acid Sequence
Price and Domestic Lead-Time Protein Production
 
Ordering
Product Description
  Analysis of N-terminal amino acid sequence (protein sequencing) is a method to excise amino acid one by one from N-terminal of protein or peptide (Edman degradation method) to be segregated by HPLC, then to detect by UV to determine amino acid sequence by the time the peak is gained (Chromatogram).

Analysis

1 Amino acid sequence analyzer is PPSQ-33A.
1 Residues possible to be analyzed is 5 to 20 residues (depending on the samples).
1 Up to the 3rd residue, possibility of analysis will be determined. Analysis will be discontinued if it was not possible because of N-terminal block or lack of sample molar mass etc. Data up to 5th residue will be taken.
1 Please note that when data was not processed because of the N-terminal block etc., there will be no analysis retry. Request for the analysis of the same sample will be accepted as a new order.

Deliverables

  Amino acid sequence identified by chromatogram, or comment in case analysis was not possible, together with a raw chromatogram data.

Price and Domestic Lead-Time
  <Price>
  Normal Analysis Rapid Analysis
Basic Fee 50,000 JPY / sample
Including analysis fee for up to 5 residues
50,000 JPY / sample + 15,000 JPY / sample (rapid fee)
Including analysis fee for up to 5 residues

* Maximum sample numbers we can accept for rapid analysis per one order are 3 samples. Please refer to the details below.

Analysis residues      Sample numbers
 10 residues or less3
 between 11 and 20 residues2
Analysis Fee 6,000JPY per residue
Charged for analysis of more than 6 residues.
When analysis was discontinued for 3 or less residues by the judgement that it was not possible, analysis fee will not be charged.
Sample Preparation Fee For liquid or dried samples:
  morecular weight of 10,000 or more: 20,000 JPY / sample
  morecular weight less than 10,000: 30,000 JPY / sample
Please send information which shows sample purity.
e.g. SDS-PAGE, Chromatogram

Fixing to PVDF membrane:
Basic charge: 50,000 JPY
Preparation: 7,000 JPY / sample
Protein having a molecular weight of 10,000 or more is acceptable.
For protein having a molecular weight less than 10,000, please inquire.
Please attach a SDS-PAGE image.
Other Service Charges Please inquire
Domestic Lead-Time Within 5~10 buiness days from sample acceptance.
* It may take more depend on sample numbers and analysis conditions.
* Orders from sample preparation would require extra business days (from one to two days depend on sample numbers).
Within 5 buiness days from sample acceptance.
* Orders from sample preparation would require extra one business day.

Regarding Sample

Sample preparation

Please refer to the below information for required sample amounts. We will discuss with customer based on PVDF membrane or SDS-PAGE image submitted with an order sheet.
* Please be noted that orders may not be acceptable depend on the amount and purity of protein.




Sample status
1 If PVDF membrane : 3 to 4 membranes of 1 mm x 8 mm
Recommended stain method : CBB-R250 staining, Ponceau S staining, Amido Black staining
* Large amount of CBB may cause some peaks around PTH-Met, Val or Pro on chromatogram and disturb PTH amino acid detection.
* Please do not use Hybond-P or nitrocellulose membrane.
1 If liquid: Please prepare for 100 ul or more sample at a concentration of 5 pmol/ul or more.
Please refer to Allowable Value of Harmful Substance In Amino Acid Sequence Analysis
1 If in a dry form (powder)
* In case sample was liquid or dried form, additional sample preparation fee will be charged.
* Please attach an Image of the sample after blotting.

Handling of Sample

Shipment condition
1 If PVDF membrane or dry form(powder) : Please use refrigerated courier of 4°C
1 If liquid : Please use refrigerated courier of - 20°C(packed with enough dry ice).

Acceptance QC of samples
We will discuss with customerabout the PVDF membrane or SDS-PAGE image submitted
by e-mail.
Any sample that was determined not to be possible to analyze is to be returned to customer at customer expense or discarded.

Left over samples
After analysis, left-over sample will be discarded.


Please refer to Allowable Value of Harmful Substance In Amino Acid Sequence Analysis

Ordering
  Please inquire.
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